The role of CD32 during HIV-1 infection

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Fig. 1: Analysis of CD4+CD32- and CD4+CD32+ populations by qVOA and proviral DNA measurements.
Fig. 2: Ultrasensitive p24 measurements.

References

  1. 1.

    Finzi, D. et al. Identification of a reservoir for HIV-1 in patients on highly active antiretroviral therapy. Science 278, 1295–1300 (1997).

    ADS  CAS  Article  Google Scholar 

  2. 2.

    Chun, T. W. et al. Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy. Proc. Natl Acad. Sci. USA 94, 13193–13197 (1997).

    ADS  CAS  Google Scholar 

  3. 3.

    Wong, J. K. et al. Recovery of replication-competent HIV despite prolonged suppression of plasma viremia. Science 278, 1291–1295 (1997).

    ADS  CAS  Article  Google Scholar 

  4. 4.

    Richman, D. D. et al. The challenge of finding a cure for HIV infection. Science 323, 1304–1307 (2009).

    ADS  CAS  Google Scholar 

  5. 5.

    Deeks, S. G. et al. Towards an HIV cure: a global scientific strategy. Nat. Rev. Immunol. 12, 607–614 (2012).

    CAS  Google Scholar 

  6. 6.

    Descours, B. et al. CD32a is a marker of a CD4 T-cell HIV reservoir harbouring replication-competent proviruses. Nature 543, 564–567 (2017).

    ADS  CAS  Google Scholar 

  7. 7.

    Laird, G. M., Rosenbloom, D. I., Lai, J., Siliciano, R. F. & Siliciano, J. D. Measuring the frequency of latent HIV-1 in resting CD4+ T cells using a limiting dilution coculture assay. Methods Mol. Biol. 1354, 239–253 (2016).

    CAS  Google Scholar 

  8. 8.

    Siliciano, J. D. et al. Long-term follow-up studies confirm the stability of the latent reservoir for HIV-1 in resting CD4+ T cells. Nat. Med. 9, 727–728 (2003).

    CAS  Google Scholar 

  9. 9.

    Eriksson, S. et al. Comparative analysis of measures of viral reservoirs in HIV-1 eradication studies. PLoS Pathog. 9, e1003174 (2013).

    CAS  Google Scholar 

  10. 10.

    Crooks, A. M. et al. Precise quantitation of the latent HIV-1 reservoir: implications for eradication strategies. J. Infect. Dis. 212, 1361–1365 (2015).

    CAS  Google Scholar 

  11. 11.

    Besson, G. J. et al. HIV-1 DNA decay dynamics in blood during more than a decade of suppressive antiretroviral therapy. Clin. Infect. Dis. 59, 1312–1321 (2014).

    CAS  Google Scholar 

  12. 12.

    Passaes, C. P. & Sáez-Cirión, A. HIV cure research: advances and prospects. Virology 454-455, 340–352 (2014).

    CAS  Google Scholar 

  13. 13.

    Pollack, R. A. et al. Defective HIV-1 proviruses are expressed and can be recognized by cytotoxic T lymphocytes, which shape the proviral landscape. Cell Host Microbe 21, 494-506.e4 (2017).

    CAS  Google Scholar 

  14. 14.

    Rosenbloom, D. I. et al. Designing and interpreting limiting dilution assays: general principles and applications to the latent reservoir for human immunodeficiency virus-1. Open Forum Infect. Dis. 2, ofv123 (2015).

    Google Scholar 

  15. 15.

    Massanella, M., Gianella, S., Lada, S. M., Richman, D. D. & Strain, M. C. Quantification of total and 2-LTR (long terminal repeat) HIV DNA, HIV RNA and herpesvirus DNA in PBMCs. Bio Protoc. 5, e1492 (2015).

    Google Scholar 

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Authors

Contributions

L.N.B., J.A.W., G.M.L., F.R.S., R.F.S. and J.D.S. designed experiments. S.A.B., C.T. and L.J.M. obtained samples. L.N.B., J.A.W., S.A.B., G.M.L., F.R.S., J.L., A.J.M., A.A.R.A. and J.D.S. performed experiments. F.R.S., H.J. and J.B.M. performed cell sorting. L.N.B., J.A.W., F.R.S., A.J.M., A.A.R.A., R.F.S. and J.D.S. analysed the data and wrote the manuscript.

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Correspondence to Robert F. Siliciano.

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Declared none.

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Supplementary Information

This file contains Supplementary Tables 1-3, Supplementary Figures 1-2 and the Supplementary Methods.

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Bertagnolli, L.N., White, J.A., Simonetti, F.R. et al. The role of CD32 during HIV-1 infection. Nature 561, E17–E19 (2018). https://doi.org/10.1038/s41586-018-0494-3

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Keywords

  • Quantitative Viral Outgrowth Assay
  • CD25hi Cells
  • Negative Depletion
  • Replication-competent Provirus
  • Standard ELISA

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