Extended Data Fig. 5: Stimulated, supported and chemoattracted mouse propriospinal axons regrow through the lesion core in contact with various substrate molecules, including putatively inhibitory CSPGs. | Nature

Extended Data Fig. 5: Stimulated, supported and chemoattracted mouse propriospinal axons regrow through the lesion core in contact with various substrate molecules, including putatively inhibitory CSPGs.

From: Required growth facilitators propel axon regeneration across complete spinal cord injury

Extended Data Fig. 5

a, Left and left middle, multi-fluorescent, detail images show BDA-labelled axons regrowing along and among surfaces decorated with fibronectin or collagen. Right middle, orthogonal 3D confocal images of the outlined areas show direct contact between BDA-labelled axons and fibronectin or collagen. Right, quantification of fibronectin or collagen levels and dot blots. Data are mean ± s.e.m. of density, n = 4 mice per group. *P < 0.01, one-way ANOVA with Bonferroni, F(3, 12) = 13.0 for fibronectin dot blot and F(3, 12) = 10.2 for collagen dot blot). b, Left and middle, multi-fluorescent, detail images show BDA-labelled axons regrowing along and among surfaces decorated with brevican (BCAN). Right, orthogonal 3D confocal image of the outlined area shows direct contact between BDA-labelled axons and BCAN. c, Multi-fluorescent, orthogonal 3D confocal images show BDA-labelled axons regrowing along and in direct contact with surfaces decorated with both CSPG4 and laminin (arrows).

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