Extended Data Fig. 7: Comparison of genomic data from astrocytes and non-astrocyte cells from mice with or without FGF + EGF after SCI. | Nature

Extended Data Fig. 7: Comparison of genomic data from astrocytes and non-astrocyte cells from mice with or without FGF + EGF after SCI.

From: Required growth facilitators propel axon regeneration across complete spinal cord injury

Extended Data Fig. 7

a, Heat maps showing significantly differentially expressed genes (DEGs) derived by RNA-seq of mRNAs from spinal cord tissue of mice treated with SCI + 1D + FGF + EGF (D + GF), and the expression of these genes in mice treated with SCI + 1D empty (D-Em), at two weeks after SCI. Data are shown for mRNAs derived selectively from astrocytes or from all other cell types (non-astrocytes), isolated as previously described19. Red, upregulated; green, downregulated; relative to SCI only. n = 3 mice per group; FDR < 0.1 for differential expression. b, Total numbers of significant DEGs in astrocytes and non-astrocytes from mice as shown in the heat map in a. Red and green numerical values indicate significantly upregulated and downregulated genes, respectively. Relative to SCI only, over 900 astrocyte genes and over 300 non-astrocyte genes were significantly up- or downregulated in mice after 12 days of growth factor treatment, which were not significantly altered by treatment with empty depots. c, Top five networks of genes significantly altered by SCI + 1D + FGF + EGF that were not altered by SCI + 1D empty after SCI relative to SCI only, as identified by unbiased analysis (Ingenuity). Full RNA-seq data are available at NCBI Gene Expression Omnibus under accession GSE111529.