Extended Data Fig. 6: BDA tract-tracing of propriospinal axon regrowth after SCI along and among different cell types. | Nature

Extended Data Fig. 6: BDA tract-tracing of propriospinal axon regrowth after SCI along and among different cell types.

From: Required growth facilitators propel axon regeneration across complete spinal cord injury

Extended Data Fig. 6

a, Multiple channel fluorescent images show the same BDA-labelled axon transitioning from contact with GFAP-positive astrocytes in proximal scar border to contact with CD13+ stromal cells in the lesion core. Numbers and arrows indicate the same locations in images of different combinations of fluorescent markers: 1, axons in contact with astrocyte processes; 2, axons in contact with both astrocyte process and stromal cell; 3, axons in contact with stromal cell. b, Multiple channel fluorescent images show axons regrowing along, and following the trajectory of, stromal cells (S) while circumventing clusters of inflammatory cells (Inf) in the lesion core. c, Multiple channel fluorescent images show axons (A) regrowing along the trajectory of blood vessels (bv) in contact with stromal cells that are present on endothelia (E) positive for platelet endothelial cell adhesion molecule (PECAM). d, Multiple channel fluorescent images show BDA-labelled propriospinal axons and cells expressing the combinatorial Schwann cell markers, p75 and SOX10, in the lesion core. Some stromal cells expressing only SOX10 but not p75 are also visible. Numbers and arrows indicate the same locations in images of different combinations of fluorescent markers: 1, axons in partial contact with cells expressing Schwann cell markers; 2, axons not in detectable contact with Schwann cells. Note that some axons are partially in contact with, and partially not in contact with, Schwann cells in lesion core.