Extended Data Fig. 3: DYRK3 inhibition leads to the formation of aberrant mitotic granules. | Nature

Extended Data Fig. 3: DYRK3 inhibition leads to the formation of aberrant mitotic granules.

From: Kinase-controlled phase transition of membraneless organelles in mitosis

Extended Data Fig. 3

a, Dissolved staining of a splicing-speckle marker (SC35) in mitotic cells upon DMSO treatment. b, Dissolved staining of stress-granule marker (PABP) in mitotic cells upon DMSO treatment. c, Spindle-pole localization of pericentriolar material protein (PCM1) in mitotic cells upon DMSO treatment. d, Mitotic cells (HEK293T) show formation of SC35 granules upon GSK-626616 treatment (1 μM, 1 h). e, Formation of SON granules in mitotic cells upon GSK-626616 treatment (1 μM, 6 h). f, Formation of TIAR granules in mitotic cells upon GSK-626616 treatment (1 μM, 6 h). g, Left, quantification of fraction of mitotic (metaphase) cells with SC35 granules on treatment with kinase inhibitors (1 μM, 1 h). Right, quantification of fraction of mitotic (metaphase) cells with PABP granules on treatment with kinase inhibitors (1 μM, 3 h). Inhibitors and the corresponding kinase targets are mentioned. Data are mean ± s.d. from three technical replicates. Images are representative of at least three independent experiments. Scale bars, 10 μm.

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