Extended Data Fig. 10: DYRK3 forms ubiquitin-positive aggregates on overexpression of CDC20 and CDH1 or upon proteosomal inhibition. | Nature

Extended Data Fig. 10: DYRK3 forms ubiquitin-positive aggregates on overexpression of CDC20 and CDH1 or upon proteosomal inhibition.

From: Kinase-controlled phase transition of membraneless organelles in mitosis

Extended Data Fig. 10

a, Ubiquitin does not colocalize with overexpressed EGFP inside cells. b, Ubiquitin localizes to EGFP–DYRK3(WT) aggregates, formed on HA–CDH1 and HA–CDC20 overexpression. c, Ubiquitin does not colocalize with overexpressed EGFP upon MG-132 treatment (5 μM, 4 h). d, Ubiquitin localizes to EGFP–DYRK3(WT) granules upon MG-132 treatment (5 μM, 4 h). EGFP–DYRK3(WT) expression was induced in HeLa-FlpIn-Trex cells by adding doxycycline (500 ng ml−1, 6 h). e, Top, EGFP–DYRK3(WT) overexpression prevents re-assembly of cytosolic mCherry–SRRM1 granules during late mitosis. Bottom, EGFP–DYRK3(WT) overexpression prevents re-assembly of cytosolic splicing granules during late mitosis. Images are representative of at least three independent experiments. Scale bars, 10 μm.