Extended Data Fig. 9: Spindle apparatus defects upon DYRK3 inhibition. | Nature

Extended Data Fig. 9: Spindle apparatus defects upon DYRK3 inhibition.

From: Kinase-controlled phase transition of membraneless organelles in mitosis

Extended Data Fig. 9

a, Multiple γ-tubulin foci in mitotic cells upon GSK-626616 treatment (1 μM, 6 h). b, Top, ZNF207 localization in interphase cells (no pre-permeabilization). Bottom, ZNF207 and SC35 colocalize in Triton-X pre-permeabilized interphase cells. c, ZNF207 granules in mitotic cells upon GSK-626616 treatment (1 μM, 3 h). d, EGFP–DYRK3(WT) colocalizes with ZNF207 in mitotic cells upon GSK-626616 treatment (1 μM, 3 h). EGFP–DYRK3(WT) expression was induced in HeLa-FlpIn-Trex cells by adding doxycycline (500 ng ml−1, 6 h). Images are representative of at least three independent experiments. Scale bars, 10 μm.