Extended Data Fig. 5: Inhibition of DYRK3 does not affect all membraneless organelles in mitosis. | Nature

Extended Data Fig. 5: Inhibition of DYRK3 does not affect all membraneless organelles in mitosis.

From: Kinase-controlled phase transition of membraneless organelles in mitosis

Extended Data Fig. 5

a, Colocalization of splicing-speckle marker and poly(A) mRNA in hybrid compartments in mitotic cells upon GSK-626616 treatment (1 μM, 3 h). b, Colocalization (arrowheads) of splicing-speckle marker and EGFP–DYRK3(WT) in hybrid compartments in mitotic cells upon GSK-626616 treatment (1 μM, 2 h). c, Dissolution of P-bodies (DDX6) in mitotic cells is unaffected upon GSK-626616 treatment (1 μM, 6 h). d, Dissolution of nucleoli (fibrillarin) in mitotic cells is unaffected upon GSK-626616 treatment (1 μM, 6 h). e, Dissolution of Cajal bodies (coilin) in mitotic cells is unaffected upon GSK-626616 treatment (1 μM, 6 hrs). f, Aberrant granules formed upon GSK-626616 treatment are not ubiquitinated aggregates. Images are representative of at least three independent experiments. Scale bars, 10 μm.