Extended Data Fig. 4: Formation of mitotic granules is DYRK3 specific. | Nature

Extended Data Fig. 4: Formation of mitotic granules is DYRK3 specific.

From: Kinase-controlled phase transition of membraneless organelles in mitosis

Extended Data Fig. 4

a, Mitotic cells show no colocalization between the splicing-speckle marker (SC35) and SRPK1 upon GSK-626616 treatment (1 μM, 1 h). b, Mitotic cells show no colocalization between the splicing speckle marker (SC35) and cyclin B upon GSK-626616 treatment (1 μM, 1 h). c, Mitotic cells show no colocalization between the splicing-speckle marker (SRRM2) and CDK1 upon GSK-626616 treatment (1 μM, 1 h). d, Mitotic cells stained for pY15 CDK1. Loss of pY15 signal (CDK1 activation) in mitotic cells upon GSK-626616 (1 μM, 1 h) treatment is comparable to DMSO control. e, GSK-626616 treatment (1 μM, 1 h) does not result in a decrease in pT446 APC3 (CDK1 mitotic substrate) signal in mitotic cells compared to the DMSO control. f, Mitotic cells show staining for pT446 APC3 (CDK1 mitotic substrate). The cells were pre-permeabilized with Triton-X before fixation. pT446 APC3 signal can be observed at spindle poles for both DMSO and GSK-626616 treatment (1 μM, 1 h). g, Appearance of SC35 granules and spindle apparatus defects upon DYRK3 knockdown. Right, quantification of SC35 granule number in mitotic (metaphase) cells (four independent experiments). Box plots: centre line, population median; box, interquartile range; whiskers, 1.5× interquartile range; dots, outliers. Statistical analysis performed across cells using a Welch’s two-sided t-test. Images and data are representative of at least three independent experiments. Scale bars, 10 μm.

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