Extended Data Fig. 2: NMR spectroscopy of ddhCTP. | Nature

Extended Data Fig. 2: NMR spectroscopy of ddhCTP.

From: A naturally occurring antiviral ribonucleotide encoded by the human genome

Extended Data Fig. 2

a, 13C–13C COSY spectrum of 13C915N3–ddhCTP. The assignments for the observed correlations of the 13C-connectivities are indicated with the grey dotted lines. b, 31P NMR spectra (300 MHz) of ddhCTP (1 mM) in D2O at 300 K. Three resonance peaks at –19.5 (triplet), −9.5 (doublet) and –3.9 (doublet) p.p.m. correspond to the beta, alpha and gamma phosphates of ddhCTP, respectively. c, 2D-HSQC NMR spectra collected on purified 1 mM ddhCTP in D2O. d, 2D-HSQC NMR spectra collected on 1 mM synthetic ddhC in D2O. All experiments have been repeated twice.

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