Extended Data Fig. 3: Place preference by photostimulation of cortico-amgydalar projections. | Nature

Extended Data Fig. 3: Place preference by photostimulation of cortico-amgydalar projections.

From: The coding of valence and identity in the mammalian taste system

Extended Data Fig. 3

a, Representative tracking of a mouse during the 15-min place-preference test in a two-chamber arena; the left chamber in the diagram was coupled to stimulation of sweet cortico-amygdalar projections (see Methods for details); this animal spent over 80% of the test time in the chamber linked to stimulation of sweet projections to the amygdala. b, Quantification of preference index before (Pre) and during light stimulation. n = 8 mice, two-tailed paired t-test, P = 0.0156. c, d, Place-preference test with stimulation of bitter cortical projections in the amygdala. n = 5 mice, two-tailed paired t-test, P = 0.0207. e, f, Animals used in d were also tested in a licking assay with similar light stimulation intensity, demonstrating strong suppression of licking responses. n = 5 mice, two-tailed paired t-test, P = 0.0056. Values are mean ± s.e.m. We note that we have examined multiple independent behavioural experiments activating sweet projections to the BLA and have never observed the induction of motor patterns or consummatory behaviour. Strong stimulation of bitter cortico-amygdalar projections (20 Hz, 10–15 mW) often elicited prototypical orofacial rejection behaviour (Supplementary Video 1).

Back to article page