Extended Data Fig. 5: Directionality of TGFα and VEGF-B signalling during EAE. | Nature

Extended Data Fig. 5: Directionality of TGFα and VEGF-B signalling during EAE.

From: Microglial control of astrocytes in response to microbial metabolites

Extended Data Fig. 5

a, b, EAE development in wild-type mice injected with pGFAP-shControl, pGFAP-shErbb1 and pCD11b-shFlt1 (a), or pCD11b-shControl, pCD11b-shTgfa and pCD11b-shVegfb (b). Clinical course. n = 5 mice per group. Representative of two independent experiments with n = 5 mice per group. c, Left, flow cytometry analysis of TGFα and VEGF-B expression in astrocytes as in a. Right, quantification of cytokine-positive astrocytes. Data are mean ± s.e.m. and P values were determined by one way ANOVA followed by Tukey’s post-hoc test. Representative of two independent experiments with four biological replicates. d, Left, flow cytometry analysis of FLT-1 and ErbB1 expression in microglia as in b. Right, quantification of cell-surface receptor expression of microglia. Data are mean ± s.e.m. and P values were determined by one way ANOVA followed by Tukey’s post-hoc test. Representative of two independent experiments with four biological replicates. 

Source Data.

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