Extended Data Table 1 Extended overview of samples with reads matching HBV and PCR results

From: Ancient hepatitis B viruses from the Bronze Age to the Medieval period

  1. a, Extended overview of samples with reads matching HBV. Rows are sorted by decreasing consensus coverage. Original reads hit DIAMOND, number of sequencing reads that matched HBV using DIAMOND41; Proteins hit DIAMOND, number of HBV proteins matched by HBV sequencing reads using DIAMOND; Original reads hit BLASTn, number of sequencing reads that matched HBV using a BLASTn42 database built from dataset 3 (see Methods); Captured reads hit BLASTn, the number of reads from the capture that matched HBV using BLASTn (as above); Bit score cut-off, the bit score cut-off above which matching reads were used to form consensus sequences; coverage consensus and depth, the percentage of the consensus genome covered by matching reads and average depth of coverage across the reference genome as reported by Geneious48. When reading sample information across a row, an N/D (not determined) cell will be encountered when processing on that sample was concluded, either owing to too few matching reads or consensus coverage less than 50%. b, TaqMan PCR results. Four extracts from samples with HBV reads were selected for TaqMan PCR confirmation: two with a large proportion of HBV reads (DA222 and DA195), two with a small proportion of HBV reads (DA85 and DA89) and one with no HBV reads (DA351). HBV was detected in extracts from DA222 and DA195, whereas the three low- and zero-read samples were negative, as were all no-template controls.