Measurement of changes in intra-axonal calcium with high temporal and spatial resolution has been technically difficult. Now, a technique has been developed that enables the genetically encoded calcium indicator GCaMP6 to be transported into axons, where calcium fluctuations can be monitored with high signal-to-noise ratio, brightness and stability. This approach was used to measure layer-specific axon activity in mouse cortex at deeper cortical levels than was previously possible.
Broussard, G. J. et al. In vivo measurement of afferent activity with axon-specific calcium imaging. Nat. Neurosci. 21, 1272–1280 (2018)
About this article
Cite this article
Lewis, S. Catching waves. Nat Rev Neurosci 19, 581 (2018). https://doi.org/10.1038/s41583-018-0062-8