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Kidney metabolism

Spatial metabolic tracing in the kidney — the future is now

A new study used metabolic tracing of the three main carbon sources (glucose, glutamine and lipids) on cultured slices of mouse kidneys to identify the dynamic metabolic changes that occur after injury in different segments of the kidney tubule at the single-cell level.

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Fig. 1: Schematic overview of spatial metabolic tracing in the kidney.


  1. Doke, T. & Susztak, K. The multifaceted role of kidney tubule mitochondrial dysfunction in kidney disease development. Trends Cell Biol. 32, 841–853 (2022).

    Article  Google Scholar 

  2. Bhargava, P. & Schnellmann, R. G. Mitochondrial energetics in the kidney. Nat. Rev. Nephrol. 13, 629–646 (2017).

    Article  CAS  Google Scholar 

  3. Wang, G. et al. Analyzing cell-type-specific dynamics of metabolism in kidney repair. Nat. Metab. 4, 1109–1118 (2022).

    Article  CAS  Google Scholar 

  4. Dixon, E. E., Wu, H., Sulvarán-Guel, E., Guo, J. & Humphreys, B. D. Spatially resolved transcriptomics and the kidney: many opportunities. Kidney Int. 102, 482–491 (2022).

    Article  CAS  Google Scholar 

  5. Jang, C., Chen, L. & Rabinowitz, J. D. Metabolomics and isotope tracing. Cell 173, 822–837 (2018).

    Article  CAS  Google Scholar 

  6. Taylor, M. J., Lukowski, J. K. & Anderton, C. R. Spatially resolved mass spectrometry at the single cell: recent innovations in proteomics and metabolomics. J. Am. Soc. Mass Spectrom. 32, 872–894 (2021).

    Article  CAS  Google Scholar 

  7. Wang, L. et al. Spatially resolved isotope tracing reveals tissue metabolic activity. Nat. Methods 19, 223–230 (2022).

    Article  CAS  Google Scholar 

  8. Uchida, S. & Endou, H. Substrate specificity to maintain cellular ATP along the mouse nephron. Am. J. Physiol. 255, F977–F983 (1988).

    CAS  Google Scholar 

  9. Kirita, Y., Wu, H., Uchimura, K., Wilson, P. C. & Humphreys, B. D. Cell profiling of mouse acute kidney injury reveals conserved cellular responses to injury. Proc. Natl Acad. Sci. USA 117, 15874–15883 (2020).

    Article  CAS  Google Scholar 

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The author thanks L. Cassina and M. E. Steidl for feedback on the manuscript before submission. A.B. is supported by the Italian Ministry of Health (RF-2018-12368254; RF-2016-02361267), the Italian association of patients with PKD (AIRP), the PKD Foundation (218G18), the European Community (H-2020-MSCA-ITN-2019#SCiLS) and the Italian Association for Research on Cancer (AIRC, IG2019-23513).

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Correspondence to Alessandra Boletta.

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Boletta, A. Spatial metabolic tracing in the kidney — the future is now. Nat Rev Nephrol 19, 5–6 (2023).

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