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Pitfalls in lipid mass spectrometry of mammalian samples — a brief guide for biologists

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Publications that report mass spectrometry analyses of lipids often include lipid species that probably do not exist in the samples. Here we provide recommendations for scientists on submitting lipid data, and for reviewers, editors and readers on evaluating these data, to reduce the reporting of erroneous lipid species.

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  • 10 July 2024

    In the version of the article initially published, refs. 10 and 11 were switched and have now been amended in the HTML and PDF version of the article.

References

  1. Kofeler, H. C. et al. Recommendations for good practice in MS-based lipidomics. J. Lipid Res. 62, 100138 (2021).

    Article  PubMed  PubMed Central  Google Scholar 

  2. Skotland, T. & Sandvig, K. Need for more focus on lipid species in studies of biological and model membranes. Prog Lipid Res. 86, 101160 (2022).

    Article  CAS  PubMed  Google Scholar 

  3. Wood, P. L. & Cebak, J. E. Lipidomics biomarker studies: Errors, limitations, and the future. Biochem. Biophys. Res. Commun. 504, 569–575 (2018).

    Article  CAS  PubMed  Google Scholar 

  4. Menzel, J. P. et al. Ozone-enabled fatty acid discovery reveals unexpected diversity in the human lipidome. Nat. Commun. 14, 3940 (2023).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Sandhoff, R. Very long chain sphingolipids: tissue expression, function and synthesis. FEBS Lett. 584, 1907–1913 (2010).

    Article  CAS  PubMed  Google Scholar 

  6. Skotland, T. & Sandvig, K. The role of PS 18:0/18:1 in membrane function. Nat. Commun. 10, 2752 (2019).

    Article  PubMed  PubMed Central  Google Scholar 

  7. McDonald, J. G. et al. Introducing the lipidomics minimal reporting checklist. Nat. Metab. 4, 1086–1088 (2022).

    Article  PubMed  Google Scholar 

  8. Kofeler, H. C. et al. Quality control requirements for the correct annotation of lipidomics data. Nat. Commun. 12, 4771 (2021).

    Article  PubMed  PubMed Central  Google Scholar 

  9. Burla, B. et al. MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines. J. Lipid Res. 59, 2001–2017 (2018).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. von Gerichten, J. et al. Single-cell untargeted lipidomics using liquid chromatography and data-dependent acquisition after live cell selection. Anal. Chem. 96, 6922–6929 (2024).

    Article  Google Scholar 

  11. Vandenbosch, M. et al. Toward omics-scale quantitative mass spectrometry imaging of lipids in brain tissue using a multiclass internal standard mixture. Anal. Chem. 95, 18719–18730 (2023).

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Tore Skotland or Kim Ekroos.

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K.E. is the owner of Lipidomics Consulting Ltd. The other authors declare no competing interests.

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Nature Reviews Molecular Cell Biology thanks the anonymous reviewer(s) for their contribution to the peer review of this work.

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Lipidomics Standards Initiative: https://lipidomicstandards.org/

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Skotland, T., Ekroos, K., McDonald, J. et al. Pitfalls in lipid mass spectrometry of mammalian samples — a brief guide for biologists. Nat Rev Mol Cell Biol (2024). https://doi.org/10.1038/s41580-024-00758-4

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  • DOI: https://doi.org/10.1038/s41580-024-00758-4

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