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Protrusion-derived vesicles: new subtype of EVs?

Cellular protrusions are highly dynamic structures that facilitate cell–cell communication and are increasingly recognised for their roles in the shedding of bioactive extracellular vesicles (EVs). The intrinsic and extrinsic mechanisms that govern the shedding of EVs from cellular protrusions and their potential physiological roles are beginning to emerge.

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References

  1. van Niel, G. et al. Challenges and directions in studying cell-cell communication by extracellular vesicles. Nat. Rev. Mol. Cell Biol. 23, 369–382 (2022).

    Article  Google Scholar 

  2. Scita, G., Confalonieri, S., Lappalainen, P. & Suetsugu, S. IRSp53: crossing the road of membrane and actin dynamics in the formation of membrane protrusions. Trends Cell Biol. 18, 52–60 (2008).

    Article  CAS  Google Scholar 

  3. Nishimura, T. et al. Filopodium-derived vesicles produced by MIM enhance the migration of recipient cells. Dev. Cell 56, 842–859.e8 (2021).

    Article  CAS  Google Scholar 

  4. de Poret, A. et al. Extracellular vesicles containing the I-BAR protein IRSp53 are released from the cell plasma membrane in an Arp2/3 dependent manner. Biol. Cell https://doi.org/10.1111/boc.202100095 (2022).

    Article  Google Scholar 

  5. Gaeta, I. M., Meenderink, L. M., Postema, M. M., Cencer, C. S. & Tyska, M. J. Direct visualization of epithelial microvilli biogenesis. Curr. Biol. 31, 2561–2575.e2566 (2021).

    Article  CAS  Google Scholar 

  6. Hurbain, I. et al. Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development. Curr. Biol. 32, 361–373.e366 (2022).

    Article  CAS  Google Scholar 

  7. Thamm, K. et al. Prominin-1 (CD133) modulates the architecture and dynamics of microvilli. Traffic 20, 39–60 (2019).

    Article  CAS  Google Scholar 

  8. Inamdar, K. et al. Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53. eLife https://doi.org/10.7554/eLife.67321 (2021).

    Article  Google Scholar 

  9. Vinay, L. & Belleannée, C. EV duty vehicles: Features and functions of ciliary extracellular vesicles. Front. Genet. 13, 916233 (2022).

    Article  CAS  Google Scholar 

  10. Mathieu, M. et al. Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Nat. Commun. 12, 4389 (2021).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank N. Gov at Weizmann Institute, R. Sorkin at Tel Aviv University, and M. Kozlov at Tel Aviv University and lab members for insightful discussions. We sincerely apologize to the many authors whom we could not cite due to space limitations. This work was supported by the Fondation pour la Recherche Medicale (FRM 2020-2023), Institut Curie and the Centre National de la Recherche Scientifique (CNRS) to G.A. and G.R., Japan Society for Promotion of Science (KAKENHI, JP 20H03252, JP20KK0341, JP21H05047) to SS., Japan Society for Promotion of Science (JP20K06625) to T.N., and Japan Science and Technology cooperation CREST (JPMJCR1863) to S.S.

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Correspondence to Gisela D’Angelo or Shiro Suetsugu.

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D’Angelo, G., Raposo, G., Nishimura, T. et al. Protrusion-derived vesicles: new subtype of EVs?. Nat Rev Mol Cell Biol 24, 81–82 (2023). https://doi.org/10.1038/s41580-022-00555-x

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  • DOI: https://doi.org/10.1038/s41580-022-00555-x

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