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Reassessing the relationship between mRNA levels and protein abundance in exercised skeletal muscles

A long-standing paradigm in molecular biology assumes a direct relationship between increases in mRNA levels and the abundance of the proteins they encode. Here, we challenge our understanding of the complex relationship between changes in the skeletal muscle transcriptome and proteome in response to repeated muscle contractions.

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References

  1. Li, J. J. et al. Quantitating translational control: mRNA abundance-dependent and independent contributions and the mRNA sequences that specify them. Nucleic Acids Res. 45, 11821–11836 (2017).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  2. Vogel, C. & Marcotte, E. M. Insights into the regulation of protein abundance from proteomic and transcriptomic analyses. Nat. Rev. Genet. 13, 227 (2012).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  3. Csárdi, G. et al. Accounting for experimental noise reveals that mRNA levels, amplified by post-transcriptional processes, largely determine steady-state protein levels in yeast. PLOS Genet. 11, e1005206 (2015).

    Article  PubMed  PubMed Central  Google Scholar 

  4. Jovanovic, M. et al. Dynamic profiling of the protein life cycle in response to pathogens. Science 347, 1259038 (2015).

    Article  PubMed  PubMed Central  Google Scholar 

  5. Williams, R. S. & Neufer, P. D. Exercise: regulation and integration of multiple systems. in Handbook of Physiology. 25, 1124–1150 (Oxford University Press, 1996).

  6. Miller, B. F. et al. The rigorous study of exercise adaptations: why mRNA might not be enough. J. Appl. Physiol. 21, 594–596 (2016).

    Article  Google Scholar 

  7. Fournier, M. L. et al. Delayed correlation of mRNA and protein expression in rapamycin-treated cells and a role for Ggc1 in cellular sensitivity to rapamycin. Mol. Cell. Proteomics 9, 271–284 (2010).

    Article  PubMed  CAS  Google Scholar 

  8. Perry, C. G. et al. Repeated transient mRNA bursts precede increases in transcriptional and mitochondrial proteins during training in human skeletal muscle. J. Physiol. 588, 4795–4810 (2010).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  9. Granata, C. et al. Training-induced changes in mitochondrial content and respiratory function in human skeletal muscle. Sports Med. 48, 1809–1828 (2018).

    Article  PubMed  Google Scholar 

  10. Wadley, G. D. et al. Xanthine oxidase inhibition attenuates skeletal muscle signaling following acute exercise but does not impair mitochondrial adaptations to endurance training. Am. J. Physiol. Endocrinol. Metab. 304, E853–E862 (2013).

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

Our work is, in part, funded by an Australian Research Council grant (DP160102176).

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Correspondence to David J. Bishop.

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Nature Reviews Molecular Cell Biology thanks the anonymous reviewers for their contribution to the peer review of this work.

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Bishop, D.J., Hawley, J.A. Reassessing the relationship between mRNA levels and protein abundance in exercised skeletal muscles. Nat Rev Mol Cell Biol 23, 773–774 (2022). https://doi.org/10.1038/s41580-022-00541-3

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  • DOI: https://doi.org/10.1038/s41580-022-00541-3

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