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In silico analysis of RNA-seq requires a more complete description of methodology

Nature Reviews Molecular Cell Biology volume 20, pages 451–452 (2019)Cite this article

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The computational analysis of RNA-seq data — from raw reads to transcript quantification — requires the sequential use of specific tools and biological references, the combination of which determines the results. The combinatorial nature of the RNA-seq pipeline necessitates a complete description of all computational choices and parameters to ensure reproducibility.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Québec, Canada

    Joël Simoneau & Michelle S. Scott

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  1. Joël Simoneau
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  2. Michelle S. Scott
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Correspondence to Michelle S. Scott.

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The authors declare no competing interests.

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Related links

Functional Genomics Data Society: minimum information about a high-throughput SEQuencing experiment: http://fged.org/site_media/pdf/MINSEQE_1.0.pdf

Gene Expression Omnibus: https://www.ncbi.nlm.nih.gov/geo/

Zenodo: https://zenodo.org/

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Simoneau, J., Scott, M.S. In silico analysis of RNA-seq requires a more complete description of methodology. Nat Rev Mol Cell Biol 20, 451–452 (2019). https://doi.org/10.1038/s41580-019-0137-z

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