Choose your yeast strain carefully: the RAD5 gene matters

The commonly used budding yeast strain W303 carries a mutant RAD5 gene (rad5-535), but numerous laboratories use a strain corrected for the mutation. This has resulted in different phenotypes of W303 cells in similar experiments. Here, we aim to raise awareness of the issue to ensure data reproducibility and interpretation.

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Correspondence to Sherif F. El-Khamisy.

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Elserafy, M., El-Khamisy, S.F. Choose your yeast strain carefully: the RAD5 gene matters. Nat Rev Mol Cell Biol 19, 343–344 (2018).

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