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Choose your yeast strain carefully: the RAD5 gene matters

The commonly used budding yeast strain W303 carries a mutant RAD5 gene (rad5-535), but numerous laboratories use a strain corrected for the mutation. This has resulted in different phenotypes of W303 cells in similar experiments. Here, we aim to raise awareness of the issue to ensure data reproducibility and interpretation.

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References

  1. Xu, X. et al. Involvement of budding yeast Rad5 in translesion DNA synthesis through physical interaction with Rev1. Nucleic Acids Res. 44, 5231–5245 (2016).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  2. Fan, H. Y., Cheng, K. K. & Klein, H. L. Mutations in the RNA polymerase II transcription machinery suppress the hyperrecombination mutant hpr1 delta of Saccharomyces cerevisiae. Genetics 142, 749–759 (1996).

    PubMed  PubMed Central  CAS  Google Scholar 

  3. Conde, F. & San-Segundo, P. A. Role of Dot1 in the response to alkylating DNA damage in Saccharomyces cerevisiae: regulation of DNA damage tolerance by the error-prone polymerases Polζ/Rev1. Genetics 179, 1197–1210 (2008).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  4. Alcasabas, A. A. et al. Mrc1 transduces signals of DNA replication stress to activate Rad53. Nat. Cell Biol. 3, 958–965 (2001).

    Article  PubMed  CAS  Google Scholar 

  5. Komata, M., Bando, M., Araki, H. & Shirahige, K. The direct binding of Mrc1, a checkpoint mediator, to Mcm6, a replication helicase, is essential for the replication checkpoint against methyl methanesulfonate-induced stress. Mol. Cell. Biol. 29, 5008–5019 (2009).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

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Correspondence to Sherif F. El-Khamisy.

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CommunityW303.html: http://wiki.yeastgenome.org/index.php/CommunityW303.html

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Elserafy, M., El-Khamisy, S.F. Choose your yeast strain carefully: the RAD5 gene matters. Nat Rev Mol Cell Biol 19, 343–344 (2018). https://doi.org/10.1038/s41580-018-0005-2

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