Antibiotic resistance is a global human health threat, causing routine treatments of bacterial infections to become increasingly difficult. The problem is exacerbated by biofilm formation by bacterial pathogens on the surfaces of indwelling medical and dental devices that facilitate high levels of tolerance to antibiotics. The development of new antibacterial nanostructured surfaces shows excellent prospects for application in medicine as next-generation biomaterials. The physico-mechanical interactions between these nanostructured surfaces and bacteria lead to bacterial killing or prevention of bacterial attachment and subsequent biofilm formation, and thus are promising in circumventing bacterial infections. This Review explores the impact of surface roughness on the nanoscale in preventing bacterial colonization of synthetic materials and categorizes the different mechanisms by which various surface nanopatterns exert the necessary physico-mechanical forces on the bacterial cell membrane that will ultimately result in cell death.
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Funding from the Australian Research Council (ARC) Industrial Transformation Research Hubs scheme (project number IH130100017) and the ARC Industrial Transformation Training Centre scheme (project number IC180100005) to E.P.I. and NIH grant R01GM124436 to P.S. are gratefully acknowledged.
The authors declare no competing interests.
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Biophysical model of the cicada wing nanopillar-bacterial cell interactions: https://www.youtube.com/watch?v=KSdMYX4gqp8
Describes specially designed materials and coatings to prevent or remove biofouling by any number of organisms on wetted surfaces.
The physical property of having no affinity with water (that is, water repellent).
The action of a chemical substance or microorganism to destroy a harmful organism or to prevent or control its effects.
Functional bone adherence to the implant surface.
Guiding the reparative growth of the natural bone.
A technique used to impart physical patterns onto a surface with surface features (protrusions or depressions) on the scale of micrometres or nanometres. The size and shapes of such patterns determine the overall topography of the surface at these scales. Such patterns can be made by depositing material onto a surface, removing material from a surface, or both.
The specific surface features that form or are generated at the nanoscale.
- Surface roughness
Irregularities of the surface, either random or repetitive, that make up the 3D topography of the surface.
Physical dimensions less than 1 μm.
The action of natural or synthetic nanomaterials that can physically induce bacterial cell death through the application of physical forces.
The physical property of a molecule and/or surface the interactions of which with water are thermodynamically favourable; that is, it can form ionic or hydrogen bonds with water.
Physical dimensions less than 1 mm.
- Stretching modulus
Also known as Young’s modulus. The modulus of elasticity: a measure of the ability of a material to withstand changes in length when under lengthwise tension or compression.
- Turgor pressure
The force (hydrostatic pressure) within the cell, caused by the osmotic flow of water, that pushes the cell membrane against the cell wall.
Imitating the models, systems and elements found in nature.
- Aspect ratio
The ratio of feature width to feature height. ‘High aspect ratio’ refers to a structure that is exceedingly tall in comparison with its width.
Causing cell damage or death.
The degree to which an organic molecule can dissolve in fat, oil or other non-polar solvents.
The physical property of a molecule or compound that possesses both hydrophilic (water-loving) and hydrophobic (water-repellent) characteristics.
Engineered substances that can be introduced into body tissues as part of an implanted medical, therapeutic or diagnostic device.
The ability of a biomaterial to perform its desired function without eliciting detrimental local or systemic effects when implanted within the body.
Supportive of the common functions of all cells, including basal functions such as viability and proliferation as well as functions specific to the phenotype of each cell type defined by morphology of the cytoskeleton and expression of proteins and specific enzymes.
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Linklater, D.P., Baulin, V.A., Juodkazis, S. et al. Mechano-bactericidal actions of nanostructured surfaces. Nat Rev Microbiol 19, 8–22 (2021). https://doi.org/10.1038/s41579-020-0414-z
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