Discovering that chytrid fungi cause chytridiomycosis in amphibians represented a paradigm shift in our understanding of how emerging infectious diseases contribute to global patterns of biodiversity loss. In this Review we describe how the use of multidisciplinary biological approaches has been essential to pinpointing the origins of amphibian-parasitizing chytrid fungi, including Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans, as well as to timing their emergence, tracking their cycles of expansion and identifying the core mechanisms that underpin their pathogenicity. We discuss the development of the experimental methods and bioinformatics toolkits that have provided a fuller understanding of batrachochytrid biology and informed policy and control measures.
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We acknowledge funding from the Natural Environment Research Council (NERC) (NE/E006701/1, NE/E006841/1, NE/G002193/1, NE/K014455/1, NE/K012 509/1, NE/M000591/1, NE/N009800/1, NE/N009967/1, NE/S000844/1, NE/S000992/1), The Morris Animal Foundation (D12ZO-002 and D16ZO-022) and the Leverhulme Trust (RPG-2014-273). We thank S. O’Hanlon and P. Ghosh, who assisted with drafting the figures. M.C.F. is a Fellow in the CIFAR ‘Fungal Kingdom’ Program.
The authors declare no competing interests.
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Amphibian Disease Portal: https://amphibiandisease.org
North American Bsal Task Force: http://www.salamanderfungus.org/about-bsal/
Global outbreak of an infectious disease in animals.
- Multilocus sequence typing
Matching the DNA sequences of fragments of multiple housekeeping genes in order to assay genetic diversity.
Local outbreaks of an infectious disease in animals.
- Bayesian-based haplotype clustering
Population assignment using large numbers of resequenced genomes.
- Mutation–drift equilibrium
State of balance in which the rate at which variation is lost through genetic drift is equal to the rate at which new variation is created by mutation.
- Tajima’s D statistic
Population genetic test statistic distinguishing between DNA sequences that evolve neutrally (at mutation–drift equilibria) and those that evolve in response to a nonrandom process, such as demographic change or natural selection.
Subjected to a process of assigning alleles to the paternal and maternal chromosomes.
Segregation of alleles between homologous chromosomes through DNA breaks and reconnections.
Sexual recombination resulting in crossovers.
- Mating-type alleles
Genes that regulate compatibility leading to meiosis in fungi, also called mating-type ‘idiomorphs’.
- Chromosomal copy number variation
State in which the number of copies of a haplotype varies between one individual and another, also known as ‘aneuploidy’.
- Amphibian arks
Ex situ breeding of threatened species in biocontainment facilities.
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Fisher, M.C., Garner, T.W.J. Chytrid fungi and global amphibian declines. Nat Rev Microbiol 18, 332–343 (2020). https://doi.org/10.1038/s41579-020-0335-x
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