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Expanding dendritic cell nomenclature in the single-cell era

Single-cell technologies have enabled extensive profiling studies of human and mouse tissues and the identification of an ever-growing number of transcriptional clusters within the dendritic cell (DC) lineage. Here, we discuss the importance of differentiating cell subsets from cell states when annotating DC clusters and propose a revised nomenclature of the DC lineage that integrates experimentally validated knowledge and unbiased transcriptomic profiling results.

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References

  1. Guilliams, M. et al. Dendritic cells, monocytes and macrophages: a unified nomenclature based on ontogeny. Nat. Rev. Immunol. 14, 571–578 (2014).

    CAS  Article  Google Scholar 

  2. Zilionis, R. et al. Single-cell transcriptomics of human and mouse lung cancers reveals conserved myeloid populations across individuals and species. Immunity 50, 1317–1334 (2019).

    CAS  Article  Google Scholar 

  3. Zhang, Q. et al. Landscape and dynamics of single immune cells in hepatocellular carcinoma. Cell 179, 829–845 (2019).

    CAS  Article  Google Scholar 

  4. Dutertre, C. A. et al. Single-cell analysis of human mononuclear phagocytes reveals subset-defining markers and identifies circulating inflammatory dendritic cells. Immunity 51, 573–589 (2019).

    CAS  Article  Google Scholar 

  5. Maier, B. et al. A conserved dendritic-cell regulatory program limits antitumour immunity. Nature 580, 257–262 (2020).

    CAS  Article  Google Scholar 

  6. Villani, A. C. et al. Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes and progenitors. Science 356, eaah4573 (2017).

    Article  Google Scholar 

  7. Bourdely, P. et al. Transcriptional and functional analysis of CD1c+ human dendritic cells identifies a CD163+ subset priming CD8+CD103+ T cells. Immunity 53, 335–352 (2020).

    CAS  Article  Google Scholar 

  8. Cytlak, U. et al. Differential IRF8 transcription factor requirement defines two pathways of dendritic cell development in humans. Immunity 53, 353–370 (2020).

    CAS  Article  Google Scholar 

  9. Ardouin, L. et al. Broad and largely concordant molecular changes characterize tolerogenic and immunogenic dendritic cell maturation in thymus and periphery. Immunity 45, 305–318 (2016).

    CAS  Article  Google Scholar 

  10. Miller, J. C. et al. Deciphering the transcriptional network of the dendritic cell lineage. Nat. Immunol. 13, 888–899 (2012).

    CAS  Article  Google Scholar 

Download references

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Correspondence to Florent Ginhoux, Martin Guilliams or Miriam Merad.

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The authors declare no competing interests.

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Nature Reviews Immunology thanks S. Nutt, who co-reviewed with M. Chopin, and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.

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Ginhoux, F., Guilliams, M. & Merad, M. Expanding dendritic cell nomenclature in the single-cell era. Nat Rev Immunol 22, 67–68 (2022). https://doi.org/10.1038/s41577-022-00675-7

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  • DOI: https://doi.org/10.1038/s41577-022-00675-7

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