Neuroinflammation and neurodegeneration in human brain at single-cell resolution


Single-cell RNA sequencing and single-nucleus RNA sequencing have recently provided the opportunity to investigate cellular and molecular aspects of neuro-immune interactions in the brain with unprecedented detail. Here, we highlight the major advances in human neuroimmunology reported this year based on these cutting-edge technologies.

Key advances

  • Single-cell and single-nucleus RNA sequencing (snRNA-seq) using brain tissue from patients with multiple sclerosis revealed multiple distinct subsets of microglia; showed that the excitatory neurons of cortical layers 2-3 are most affected by the disease; and identified a subset of oligodendrocytes that express pro-inflammatory genes.

  • snRNA-seq deconvoluted progressive molecular alterations of various cell types arising during Alzheimer disease and revealed potential gender differences.

  • Epigenetic profiling of single nuclei identified enhancers that control the expression of genes underlying the identity and function of all human brain cells.

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Fig. 1: Transcriptomic changes in brain disease.


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We thank A. Swain and S. Gilfillan for helpful suggestions during the preparation of the manuscript.

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Correspondence to Marco Colonna.

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Competing interests

M.C. receives research support from Alector, Amgen and Ono. S.B. declares no competing interests.

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Colonna, M., Brioschi, S. Neuroinflammation and neurodegeneration in human brain at single-cell resolution. Nat Rev Immunol 20, 81–82 (2020).

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