Single-cell analyses in recent years have shown major differences in the transcriptome between individual cells in the same induced pluripotent stem cell-derived clones. Although these differences are in part attributable to genetic and epigenetic modifications of the nuclear genome, emerging evidence suggests that variants in mitochondrial DNA also play a pivotal role.
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Acknowledgements
V.C. is supported by the University of Bologna and the IRCCS Istituto delle Scienze Neurologiche. M.H. is supported by the National Institutes of Health (NIH U54 NS078059) and Marriott Mitochondrial Disease Clinic Research Fund (MMDCRF) from the J. Willard and Alice S. Marriott Foundation. J.A.E. is supported by the Centro de Investigación Biomédica en Red en Fragilidad y Envejecimento Saludable (CIBERFES16/10/00282) and Severo Ochoa Program for Centers of Excellence to CNIC (SEV-2015-0505). P.F.C. is a Wellcome Trust Principal Research Fellow (212219/Z/18/Z) in the Medical Research Council Mitochondrial Biology Unit (MC_UU_00015/9). This research was supported by the NIHR Cambridge Biomedical Research Centre (BRC-1215-20014). The views expressed are those of the author(s) and not necessarily those of the NIHR or the Department of Health and Social Care.
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Carelli, V., Hirano, M., Enríquez, J.A. et al. Implications of mitochondrial DNA mutations in human induced pluripotent stem cells. Nat Rev Genet 23, 69–70 (2022). https://doi.org/10.1038/s41576-021-00430-z
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DOI: https://doi.org/10.1038/s41576-021-00430-z
