Schep et al. show that non-homologous end joining is strongly favoured in euchromatin, whereas single-stranded template repair is moderately preferred in heterochromatin. Importantly, heterochromatin features that promote microhomology-mediated end joining also decrease Cas9 efficiency.
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Original article
Schep, R. et al. Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance. Mol. Cell https://doi.org/10.1016/j.molcel.2021.03.032 (2021)
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Barranco, C. Chromatin context affects DNA repair pathway. Nat Rev Genet 22, 414 (2021). https://doi.org/10.1038/s41576-021-00371-7
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DOI: https://doi.org/10.1038/s41576-021-00371-7
