Cholangiopathies account for a remarkable proportion of liver transplantation cases, reinforcing the need for novel therapeutic approaches. Organoids hold great promise as novel cell-based therapies, although their applicability has not been addressed in humans. Now, a new study has reported the therapeutic effectiveness of cholangiocyte organoids to repair the injured human biliary epithelium.
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Muff, M. A. et al. Development and characterization of a cholangiocyte cell line from the PCK rat, an animal model of Autosomal Recessive Polycystic Kidney Disease. Lab. Invest. 86, 940–950 (2006).
Banales, J. M. et al. Hepatic cystogenesis is associated with abnormal expression and location of ion transporters and water channels in an animal model of autosomal recessive polycystic kidney disease. Am. J. Pathol. 173, 1637–1646 (2008).
Sato, T. et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459, 262–265 (2009).
Sampaziotis, F. et al. Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids. Nat. Med. 23, 954–963 (2017).
Huch, M. et al. Long-term culture of genome-stable bipotent stem cells from adult human liver. Cell 160, 299–312 (2015).
Sampaziotis, F. et al. Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nat. Biotechnol. 33, 845–852 (2015).
Huch, M. et al. In vitro expansion of single Lgr5 + liver stem cells induced by Wnt-driven regeneration. Nature 494, 247–250 (2013).
Nuciforo, S. & Heim, M. H. Organoids to model liver disease. JHEP Rep. 3, 100198 (2021).
Sampaziotis, F. et al. Cholangiocyte organoids can repair bile ducts after transplantation in the human liver. Science 371, 839–846 (2021).
Roos, F. J. M. et al. Human bile contains cholangiocyte organoid-initiating cells which expand as functional cholangiocytes in non-canonical Wnt stimulating conditions. Front. Cell Dev. Biol. 8, 630492 (2020).
Spanish Carlos III Health Institute (ISCIII) (J.M.B.: FIS PI15/01132, PI18/01075 and Miguel Servet Program CON14/00129 and CPII19/00008) co-financed by “Fondo Europeo de Desarrollo Regional” (FEDER); CIBERehd (ISCIII): J.M.B., P.M.R.; IKERBASQUE, Basque foundation for Science (J.M.B.), Spain; BIOEF (Basque Foundation for Innovation and Health Research: EiTB Maratoia BIO15/CA/016/BD to J.M.B.), Department of Health of the Basque Country (J.M.B.: 2017111010) and Euskadi RIS3 (J.M.B.: 2016222001, 2017222014, 2018222029; 2019222054, 2020333010); Department of Industry of the Basque Country (Elkartek: KK-2020/00008 to J.M.B.). La Caixa Scientific Foundation (J.M.B.: HR17-00601). “Fundación Científica de la Asociación Española Contra el Cáncer” (AECC Scientific Foundation, to J.M.B.). P.M.R. was funded by the ISCIII (“Sara Borrell” fellowship CD19/00254).
The authors declare no competing interests.
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Rodrigues, P.M., Banales, J.M. Applications of organoids in regenerative medicine: a proof-of-concept for biliary injury. Nat Rev Gastroenterol Hepatol 18, 371–372 (2021). https://doi.org/10.1038/s41575-021-00459-9