Microbial DNA signature in plasma enables cancer diagnosis

Bacteria within tumours affect progression and response to therapy; in addition, bacterial DNA can be detected in cell-free plasma. Herein, we discuss evidence showing that intratumoural bacteria are characteristic for each tumour type, and that detection of cell-free bacterial DNA in blood could provide an accurate and non-invasive test for cancer diagnosis.

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Fig. 1: Microbial DNA signatures in tissues and blood to identify presence and type of cancer.


  1. 1.

    Cohen, J. D. et al. Detection and localization of surgically resectable cancers with a multi-analyte blood test. Science 359, 926–930 (2018).

    CAS  Article  Google Scholar 

  2. 2.

    Poore, G. D. et al. Microbiome analyses of blood and tissues suggest cancer diagnostic approach. Nature 579, 567–574 (2020).

    CAS  Article  Google Scholar 

  3. 3.

    Dzutsev, A. et al. Microbes and cancer. Annu. Rev. Immunol. 35, 199–228 (2017).

    CAS  Article  Google Scholar 

  4. 4.

    Bullman, S. et al. Analysis of Fusobacterium persistence and antibiotic response in colorectal cancer. Science 358, 1443–1448 (2017).

    CAS  Article  Google Scholar 

  5. 5.

    Geller, L. T. et al. Potential role of intratumor bacteria in mediating tumor resistance to the chemotherapeutic drug gemcitabine. Science 357, 1156–1160 (2017).

    CAS  Article  Google Scholar 

  6. 6.

    Peters, B. A. et al. The microbiome in lung cancer tissue and recurrence-free survival. Cancer Epidemiol. Biomarkers Prev. 28, 731–740 (2019).

    Article  Google Scholar 

  7. 7.

    Riquelme, E. et al. Tumor microbiome diversity and composition influence pancreatic cancer outcomes. Cell 178, 795–806 (2019).

    CAS  Article  Google Scholar 

  8. 8.

    Kowarsky, M. et al. Numerous uncharacterized and highly divergent microbes which colonize humans are revealed by circulating cell-free DNA. Proc. Natl Acad. Sci. USA 114, 9623–9628 (2017).

    CAS  Article  Google Scholar 

  9. 9.

    Kwong, T. N. Y. et al. Association between bacteremia from specific microbes and subsequent diagnosis of colorectal cancer. Gastroenterology 155, 383–390 (2018).

    Article  Google Scholar 

  10. 10.

    Cancer Microbiome. Kraken-Derived Normalized Microbial Abundances in TCGA http://cancermicrobiome.ucsd.edu/CancerMicrobiome_DataBrowser/ (2020).

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Correspondence to Giorgio Trinchieri.

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Dzutsev, A., Trinchieri, G. Microbial DNA signature in plasma enables cancer diagnosis. Nat Rev Clin Oncol 17, 453–454 (2020). https://doi.org/10.1038/s41571-020-0391-1

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