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Validation of plasma-derived small extracellular vesicles as cancer biomarkers

A role for extracellular vesicles and particles (EVPs) as cancer biomarkers has been elusive. A mass spectrometry-based comparative analysis of EVPs from individuals with or without cancer has now enabled the identification of tumour-associated protein profiles in EVPs in plasma and paired tumour tissues, validating the role of EVPs as a novel liquid biopsy approach in cancer diagnosis.

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  1. Theodoraki, M. N. et al. Significance of PD-L1( + ) exosomes in plasma of head and neck cancer patients. Clin. Cancer Res. 24, 896–905 (2018).

    Article  CAS  Google Scholar 

  2. Chen, G. et al. Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response. Nature 560, 382–386 (2018).

    Article  CAS  Google Scholar 

  3. Theodoraki, M. N. et al. Circulating exosomes measure responses to therapy in head and neck cancer patients treated with cetuximab, ipilimumab, and IMRT. Oncoimmunology 8, 1593805 (2019).

    Article  Google Scholar 

  4. Whiteside, T. L. The potential of tumor-derived exosomes for noninvasive cancer monitoring: an update. Expert Rev. Mol. Diagn. 18, 1029–1040 (2018).

    Article  CAS  Google Scholar 

  5. Kalluri R. The biology and function of exosomes in cancer. J. Clin. Invest. 126, 1208–1215

  6. Choi, D. et al. Mapping subpopulations of cancer cell-derived extracellular vesicles and particles by nano-flow Cytometry. ACS Nano 13, 10499–10511 (2019).

    Article  CAS  Google Scholar 

  7. Buscail, E. et al. High clinical value of liquid biopsy to detect circulating tumor cells and tumor exosomes in pancreatic ductal adenocarcinoma patients eligible for up-front surgery. Cancers (Basel) 11, 1656 (2019).

    Article  CAS  Google Scholar 

  8. Jerez, S. et al. Proteomic analysis of exosomes and exosome-free conditioned media from human osteosarcoma cell lines reveals secretion of proteins related to tumor progression. J. Cell Biochem. 118, 351–360 (2017).

    Article  CAS  Google Scholar 

  9. Hoshino, A. et al. Extracellular vesicle and particle biomarkers define multiple human cancers. Cell 182, 1044–1061 (2020).

    Article  CAS  Google Scholar 

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T.L.W.’s research is supported in part by NIH grants R01 CA168626, U01 DE029759 and SBIR 75N91019C00042.

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Correspondence to Theresa L. Whiteside.

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The author declares no competing interests.

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Whiteside, T.L. Validation of plasma-derived small extracellular vesicles as cancer biomarkers. Nat Rev Clin Oncol 17, 719–720 (2020).

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