In 2017, an autologous chimeric antigen receptor (CAR) T cell therapy indicated for children and young adults with relapsed and/or refractory CD19+ acute lymphoblastic leukaemia became the first gene therapy to be approved in the USA. This innovative form of cellular immunotherapy has been associated with remarkable response rates but is also associated with unique and often severe toxicities, which can lead to rapid cardiorespiratory and/or neurological deterioration. Multidisciplinary medical vigilance and the requisite health-care infrastructure are imperative to ensuring optimal patient outcomes, especially as these therapies transition from research protocols to standard care. Herein, authors representing the Pediatric Acute Lung Injury and Sepsis Investigators (PALISI) Network Hematopoietic Stem Cell Transplantation (HSCT) Subgroup and the MD Anderson Cancer Center CAR T Cell Therapy-Associated Toxicity (CARTOX) Program have collaborated to provide comprehensive consensus guidelines on the care of children receiving CAR T cell therapy.
Leukaemias are the most common childhood cancers, and acute lymphoblastic leukaemia (ALL) is the most common form of childhood leukaemia1. In the century following its first description, leukaemia remained a near uniformly fatal disease. In 1948, astute observations among children with leukaemia, made by Sidney Farber2, led to therapeutic breakthroughs — ushering in an era of hope. Since then, paediatric oncology cooperative group trials and pioneering work by E. Donnall Thomas on haematopoietic stem cell transplantation (HSCT) among patients with leukaemia have led to dramatic improvements in the cure rates of childhood ALL3,4. Despite improvements in overall remission rates (ORRs) for children with ALL, until now, the cure rates for children with relapsed and/or refractory disease remain disappointingly low5,6. In the 1970s, E. Donnall Thomas considered a 15% salvage rate after disease relapse to be a promising result among a patient population with previously even more dismal outcomes7. Since that time, durable remission rates have increased, with other dramatic improvements in supportive care, such as antimicrobial prophylaxis and therapy, infection control, HLA tissue typing, immunosuppression, transfusion support, and paediatric critical care management, resulting in improved patient survival.
In 2017, the FDA approved the first chimeric antigen receptor (CAR) T cell therapy, tisagenlecleucel, which has been associated with ORRs of almost 90% among children and young adults with B cell precursor ALL that is treatment refractory or in second or later relapse8,9. Notably, this novel genetically engineered, CD19-targeted, autologous T cell immunotherapy was approved specifically for a paediatric and young adult indication. While CAR T cell immunotherapy is an exciting paradigm shift in anticancer therapy, this treatment modality is associated with unique toxicities, which can lead to very rapid and life-threatening cardiorespiratory and/or neurological deterioration. Thus, this novel therapy requires the medical vigilance of a diverse multidisciplinary team — and the associated comprehensive clinical infrastructure — to ensure optimal outcomes.
CAR T cells are generated through genetic modification of the patient’s own (autologous) T cells or those of an allogeneic donor. The isolated cells are activated and genetically modified via viral transduction or nonviral gene transfer10,11. Specifically, the modified CAR T cells express an engineered chimeric cell-surface receptor comprising an extracellular antigen-recognition domain, which is usually an antibody single-chain variable fragment (scFv), linked to at least one intracellular signalling domain — usually the CD3ζ chain of the T cell receptor plus one or more domains derived from co-stimulatory receptors (such as CD28 or 4-1BB ligand receptor (4-1BB; also known as TNFRSF9)). The extracellular portion of the CAR enables recognition of a specific antigen (such as CD19), and the signalling domains stimulate T cell proliferation, cytolysis, and cytokine secretion to enable elimination of the target cell12,13. Autologous or allogeneic cells genetically engineered to express CARs targeting certain molecules commonly presented on the surface of cancer cells have been associated with durable remissions among patients for whom no alternative therapies were effective14,15,16,17,18,19,20,21,22,23,24,25,26,27.
Cytokine-release syndrome (CRS) and CAR T cell-related encephalopathy syndrome (CRES) are well-described unique toxicities associated with CAR T cells and some other immunotherapies28,29,30,31,32,33; however, the pathophysiological mechanisms of both CRS and CRES remain poorly understood. Results from animal studies reported in May 2018 implicate recipient monocyte-derived and/or macrophage-derived IL-1, IL-6, and nitric oxide (and not CAR T cell-derived cytokines) as the key determinants of the severity of CRS and CRES34,35. Indeed, CRS is a systemic inflammatory response caused by the CAR T cells and involving other immune cells that is typically characterized by fever, hypoxia, tachycardia, hypotension, and multi-organ dysfunction36. CRES can occur concurrently with CRS, following its resolution, or without associated CRS and is characterized by encephalopathy, delirium, seizures, and, rarely, cerebral oedema37. Almost half of all patients who receive tisagenlecleucel require intensive monitoring and critical care support, predominantly owing to these toxicities9,38. CRS and CRES are generally reversible but can be fatal. Paediatric-specific management guidelines, comprehensive training of interdisciplinary staff, effective communication, and an appropriately phased infrastructure to ensure that adequate resources are available should facilitate the early diagnosis and appropriate management of paediatric patients who develop CRS and/or CRES after receiving CAR T cell therapy as a standard of care or according to a research protocol — and thereby achieve optimal outcomes. Herein, we provide consensus guidelines for the use of CAR T cells in paediatric patients with cancer.
A panel of experts from the HSCT Subgroup of the Pediatric Acute Lung Injury and Sepsis Investigators (PALISI) Network, the CAR T Cell Therapy-Associated Toxicity (CARTOX) Program at The University of Texas MD Anderson Cancer Center (Houston, TX, USA), and several other institutions with extensive interest and experience in CAR T cell therapy was convened to develop these guidelines. The PALISI Network includes clinical researchers from >90 paediatric intensive-care units across North America and beyond. The PALISI HSCT Subgroup is dedicated to improving the health and survival of children who require critical care during and following HSCT and cellular therapy through collaborative clinical and translational research. The guideline panel comprised a multidisciplinary and inter-professional team, including physicians with expertise in HSCT for paediatric and adult patients, translational immunotherapy researchers, paediatric intensivists, neurologists, paediatric nurses, advanced-practice providers, pharmacists, clinical nurse specialists and nursing administrators, and health-care administrators from a diverse group of medical centres. Using a modified Delphi method, the panel aimed to provide comprehensive guidelines for the safe administration of various CAR T cell therapies (standard of care and/or research protocol administration), the early recognition of CRS and/or CRES, and the management of these toxicities39. Levels of evidence and strength of recommendations were based on the classification schemes described by Shekelle and colleagues40 (Supplementary Table 1). A summary of our key recommendations and the associated level of supporting evidence are provided in Table 1.
Patient selection and evaluation
Currently, CAR T cell therapies are used mainly among patients with relapsed and/or refractory haematological cancers or other high-risk malignancies. As new genetically modified cellular therapies are developed, differences in a range of variables related to the source cell type (T cells, natural killer (NK) cells, natural killer T cells, and cytokine-induced killer cells, among others), the engineered product (for example, the co-stimulatory domains and gene transfer technologies used), the manufacturing process and reagents, the primary disease, and host factors might influence the individual toxicity profiles. Thus, providers of these treatments are encouraged to adhere to product information labels and guidance from risk evaluation and mitigation strategy (REMS) programmes as the products are approved by the FDA (Table 1).
Patient selection should be based upon the FDA-approved indications and eligibility criteria used in pivotal studies but could potentially be tailored on the basis of emerging information relating to each new product (such as label updates). Patients should generally have an acceptable performance status according to the thresholds defined in treatment protocols and/or institutional guidelines, which might vary for different indications and products and depending on whether any deficiencies are secondary to disease-specific manifestations and thus likely to improve with primary disease response. Patients should be evaluated for uncontrolled infections, active graft-versus-host disease (GVHD), or recent donor-lymphocyte infusion (DLI), with a threshold of at least 6 weeks between DLI and CAR T infusion38. Patients with uncontrolled infection and active grade II–IV acute or extensive chronic GVHD should be excluded. In patients with prior GVHD, the GVHD must have resolved and the patient should not have received systemic immunosuppression. Consideration should be given to the sites of active disease and specifically to whether immune activation, such as tumour flare ‘pseudoprogression’, could compromise vital organ function (for example, of the airway or central nervous system (CNS)). Patients with active CNS pathology should be selected with caution, particularly for products associated with CRES41,42,43. When feasible, baseline evaluation by interdisciplinary team members, such as intensive-care physicians and neurologists, could help to guide patient selection.
As new products are introduced, programmes should establish minimum eligibility criteria for patients to receive each agent. These criteria could potentially be adjusted over time on the basis of published experience gained in larger cohorts of patients. Patients identified as candidates for CAR T cell therapy should rapidly be referred for financial counselling to avoid delays in accessing care related to insurance pre-authorizations. For convenience, we have provided an overview of our general recommendation relating to CAR T cell-therapy eligibility and monitoring evaluations in the form of a checklist (Box 1).
Informed consent and assent
CAR T cell therapy is a potentially curative treatment but is associated with life-threatening toxicities and can require long-term local follow-up evaluations and restrictions. Thus, detailed informed consent for therapy should be obtained from the patient and/or their guardians; when appropriate, child assent should also be obtained44 (Table 1). Incorporation of child life and psychological services in assent discussions might be helpful. In addition, capable patients should be asked to consider age-appropriate advanced medical directives. Even before leukapheresis is performed, the patient and/or their guardians should be informed about the potential benefits of CAR T cell therapy, as well as the potential toxicities and other risks associated with the procedure. Consent for CAR T cell manufacturing and therapy should encompass leukapheresis, lymphodepletion therapy, CRS and CRES, and the potential need for bridging chemotherapy, intensive-care support (including intubation and mechanical ventilation, vasopressor and/or inotropic support, renal replacement therapy, and intracranial hypertension management after transfer to the intensive-care unit), and anti-IL-6 therapy38. Patients should be aware that, even if the CAR T cell product is manufactured successfully, infusion of the product is contingent upon continued clinical eligibility. Patients should also be informed of the need to remain within 2 hours of the treatment facility for at least 4 weeks following infusion and of any other special precautions and limitations required during post-infusion monitoring after treatment with specific products (for example, limitation of driving)8.
Leukapheresis for CAR T cell production
Generation of autologous CAR T cells requires the collection of CD3+ lymphocytes from the patient through leukapheresis. Absolute lymphocyte count (ALC) thresholds to proceed with leukapheresis can vary between different CAR T cell products; although an ALC of >100 cells/µl can be acceptable, >500 cells/µl (or an absolute CD3+ lymphocyte count of >150 cells/µl) is generally preferred45,46. Patients should undergo pre-collection testing to ensure that they are medically eligible to proceed with leukapheresis. Institutional or protocol-specific guidelines should outline haematological and other clinical criteria to proceed with collection. Paediatric patients can require a leukapheresis central venous catheter, rather than a peripheral venous cannula, for collection and should be haemodynamically stable (that is, able to tolerate fluid shifts) and free of uncontrolled infection47,48,49. The presence of infectious disease markers — for active or latent hepatitis B virus (HBV), active hepatitis C virus (HCV), or HIV — is generally assessed as part of the clearance protocol for leukapheresis. Close monitoring for hypotension, hypocalcaemia, and catheter-related pain is imperative during paediatric leukapheresis, particularly among infants and younger children who might not verbalize symptoms50,51,52,53,54 (Table 1). Packed red blood cells (irradiated) and/or albumin can be used to prime the collection in children weighing <30 kg. The targeted cell dose for leukapheresis can vary depending on the specific product and manufacturing process. Once an adequate quantity of cells has been collected, the cells are sent to the laboratory for CAR T cell manufacturing — a process that typically takes 2–4 weeks8. Cell-therapy production, storage, transportation, and shipping should occur in compliance with the most current standards as defined by the Foundation of Accreditation of Cellular Therapy (FACT)55.
Allogeneic CAR T cells
Limitations of autologous CAR T cell therapy include the time required after leukapheresis to manufacture the cell product (especially among patients with advanced-stage disease and a very narrow therapeutic window), the cost of manufacturing a patient-specific product, and the fact that adequate leukapheresis might not always be possible among heavily pretreated patients. Allogeneic CAR cells would presumably offer an ‘off-the-shelf’, third-party approach to CAR-based cell therapy56,57,58,59. CAR-transduced cord blood-derived NK cells have been reported to have potent anticancer effects in preclinical studies60. This allogeneic NK cell approach to CAR cell therapy might also be associated with a reduced risk of GVHD compared with the use of allogeneic T cells. Moreover, the NK cell product used included a ‘suicide’ gene — inducible caspase 9 — that could be pharmacologically activated to eliminate the transduced cells, thus providing an additional safety mechanism60. A clinical trial to test the safety and efficacy of this product is currently underway at the MD Anderson Cancer Center (NCT03056339). In other allogeneic CAR-expressing cell-therapy approaches, functional co-expression of RQR8, a construct combining epitopes from CD34 and CD20, renders the CAR cells sensitive to the monoclonal anti-CD20 antibody rituximab, as a safety feature56,61. Safety mechanisms designed to eliminate transduced cells have also been added to autologous CAR-expressing cells, such as anti-CD19 CAR T cells also expressing inactive, truncated EGFR, which enables the cells to be targeted through administration of the anti-EGFR antibody cetuximab49. The recommendations provided herein should be applicable to both autologous and allogeneic CAR-based cell therapies.
Some patients with high-risk advanced-stage malignancies require bridging chemotherapy in the period immediately following leukapheresis. The goal of bridging chemotherapy is to maintain disease control and prevent progression (in addition to potentially decreasing tumour burden, which might reduce the risk of severe CRS20,29,62) while the autologous CAR T cells are manufactured rather than to act as a primary treatment of the disease20,62. During this 2–4-week period, patients should be monitored for tumour-lysis syndrome (TLS) and should receive antimicrobial prophylaxis with routine infection precautions63. Indeed, the bridging chemotherapy regimen should be selected carefully during this critical period in order to minimize the risk of toxicities, which might disqualify the patient from proceeding to lymphodepletion and/or CAR T cell infusion45. Several bridging chemotherapy regimens are commonly used in paediatric patients (Box 2); however, insufficient data are currently available to recommend an optimal regimen. This choice might be influenced by certain study protocol guidelines, characteristics of the particular CAR T cell product, and/or patient-specific clinical variables (such as prior response to particular chemotherapeutic agents and baseline organ function).
Preparative lymphodepletion treatment
Several lymphodepletion regimens are commonly used in the treatment of paediatric patients9,22,64 (Box 3). The T cell pool is subject to homeostatic regulation; therefore, a lymphopenic environment might be favourable for adoptive T cell transfer owing to reduced competition for homeostatic factors. Indeed, the removal of ‘cytokine sinks’ increases the availability of cytokines that promote lymphocyte proliferation and survival, such as IL-7 and IL-15, and can be a contributing factor to the effectiveness of tumour-specific T cells65,66,67. Furthermore, the depletion of immunosuppressive CD4+CD25+ regulatory T cells68,69,70,71,72,73,74,75 has been proposed as a key mechanism by which lymphodepletion augments adoptive T cell therapy76,77. Accordingly, lymphodepletion has also been shown to improve the expansion and persistence of adoptive CAR T cells and to enhance their anticancer efficacy, resulting in increased ORRs64,66. Of note, the majority of paediatric patients enrolled in the trials of tisagenlecleucel completed lymphodepletion before CAR T cell infusion9,38. We recommend that all patients undergo lymphodepletion if possible (omission of lymphodepletion might need to be considered in patients with lymphopenia).
Cyclophosphamide-based lymphodepletion regimens are commonly used before CAR T cell infusion, and the addition of fludarabine to lymphodepletion chemotherapy has been associated with improved CAR T cell expansion and persistence and prolongation of disease-free survival in patients with ALL64,78. Alternative lymphodepletion regimens (Box 3) can be considered in patients with haemorrhagic cystitis and/or resistance to a prior cyclophosphamide-based regimen9,46,64,79,80,81 (Table 1).
To proceed with lymphodepletion and CAR T cell infusion, patients should not have uncontrolled infection because hyperinflammatory states pre-infusion have been associated with increased risks of morbidity and mortality49,82,83,84. Thus, an interval assessment should be performed on the day of initiation of the lymphodepletion regimen to identify any new complications. This evaluation usually includes screening for signs and symptoms of active infection and/or new organ toxicity, as well as exclusion of pregnancy if indicated (Box 1). Anticipatory guidance regarding the adverse effects of the specific drugs and treatment modalities used should be reviewed. Haemodynamic and laboratory monitoring and hydration should be tailored on the basis of the selected lymphodepletion regimen.
Patients should not have evidence of uncontrolled infection and/or other contraindications before CAR T cell infusion. Contraindications include active or latent HBV infection, active HCV or HIV infection, severe acute or chronic extensive GVHD, and pregnancy8,37,38,49,82,83,84,85. If any of these features are present, CAR T cell infusion should be delayed to avoid potentially severe immune activation and associated sequelae.
At the time of infusion, oxygen, suction, and emergency medications, including adrenaline, should be readily available (Box 1). The patient and/or their caregiver should be instructed to report symptoms, such as shortness of breath, rash, chills, chest pain, and back pain. Infusion should occur through the largest patent lumen without a filter and generally without an infusion pump. Pre-medication with drugs including acetaminophen and diphenhydramine should be administered 30–60 mins before CAR T cell infusion8 in order to prevent infusion reactions related to cryopreservants, such as dimethyl sulfoxide. Corticosteroids should not be routinely used for pre-medication, as these agents are lymphocytotoxic and, thus, their administration early in the treatment course (before CAR T cell expansion in vivo) could affect therapeutic outcomes20. With the use of CAR T cell products approved by the FDA, we recommend following the FDA-approved package labelling; otherwise, protocol-specific guidelines should be followed. Vital signs and urine output should be monitored closely after the time of infusion.
Infusion of cellular therapy products is generally safe, although serious adverse infusion reactions can occur. Characteristic adverse reactions include nausea, vomiting, abdominal pain, chills, fever, and, rarely, severe respiratory depression, neurotoxicity, and cardiac arrhythmias86,87,88,89,90,91,92,93. General management principles for infusion reactions associated with adoptive cell therapy include consideration of slowing or halting the infusion, activation of emergency precautions, and confirmation of product details for accuracy. If symptoms resolve uneventfully without medical intervention, a serious infusion-related event is unlikely to have occurred. If an infusion-related event is considered likely, a transfusion-reaction laboratory evaluation and appropriate supportive care according to institutional guidelines should be initiated94. Bacterial infusion reactions occur from infusion of contaminated products, typically with Gram-negative organisms; therefore, prompt treatment with appropriate antibiotics and supportive care for fever, severe hypotension, and other unexpected signs and symptoms is important to preclude clinical deterioration and potentially death.
Inpatient and outpatient management
Early recognition of toxicities of CAR T cell therapy, particularly CRS and/or CRES, in paediatric patients requires detection of variations from baseline in characteristics including heart rate, blood pressure, temperature, and irritability (Box 1). Both the efficacy and toxicity profiles of CAR T cell therapy might vary depending on the specific product administered and individual patient characteristics. Thus, the decision on inpatient versus outpatient management of patients treated with CAR T cell therapy should involve consideration of the toxicity profile of the product used, the clinical status of the patient (including risk of TLS), and the ability of the institution to deliver prompt and comprehensive outpatient management, as well as the ability of the patient to access such care. Given the potential for rapid clinical deterioration, if CAR T cell therapy is administered in an outpatient setting, a low threshold should be used for patient admission upon development of fever and/or other signs or symptoms that are suggestive of CRS and/or CRES38 (Table 1). The presence of a reliable, consistent, and well-informed caregiver is essential to facilitate outpatient administration of CAR T cell therapy. In instances in which patients are not admitted for CAR T cell infusion and CRS–CRES monitoring, adequate outpatient facilities are needed, with extended outpatient hours (as defined by the institutions), prompt access to emergency and critical care, and trained staff who are knowledgeable of CAR T cell toxicity and are capable of prompt patient evaluation and management available at all times. This requires adequate outpatient space with a design appropriate to the protection of patients who are immunocompromised while they are being triaged. Moreover, rapid access to a cellular therapy physician, and pharmacy, laboratory, and transfusion medicine services should be guaranteed.
As products transition from research study protocols to standard-of-care administration, institutional guidelines should consider whether most patients require inpatient hospitalization during the pivotal trials and/or what infrastructure is needed for outpatient administration. For example, during the international phase II ELIANA study of tisagenlecleucel in paediatric and young adult patients with CD19+ relapsed and/or refractory B cell ALL38, 76% of patients underwent cell infusions in the inpatient setting. CRS occurred in 77% of patients, with a median time to onset of 3 days (range 1–22 days)38. Almost half of all patients required intensive-care support, with a median stay of 7 days (range 1–34 days); intensive-care support included the use of high-dose vasopressors, oxygen supplementation, mechanical ventilation, and/or dialysis38. Neurological events occurred in 40% of patients, with the majority of cases occurring concurrently with, or soon after resolution of, CRS38. Two deaths occurred within 30 days of CAR T cell infusion (one patient died of cerebral haemorrhage and another died of progressive leukaemia)38. On the basis of the published experience with this product, considering inpatient admission for a minimum of 3–7 days following infusion is reasonable, especially as this treatment is increasingly being offered as a standard of care8,38 (Table 1). Nevertheless, the length of inpatient hospitalization and/or need for daily outpatient assessments might vary on the basis of the risks of CRS and CRES, the clinical and developmental status of the patient, and the social support systems available to the patient at home. The risk of developing CRS and/or CRES probably depends on the patient, the source of immune effector used to manufacture the CAR T cell therapy, the specific CAR T cell product, and/or the associated lymphodepletion strategy.
Admission orders and handoff communication should include information regarding the patient’s baseline heart rate, blood pressure, and mood, cognition, and developmental status. In addition to the aforementioned screening (Box 1), continuous cardiac — and, if feasible, telemetry — monitoring should be strongly considered, beginning on the day of CAR T cell infusion (to ascertain baseline physiology) and continuing for a minimum number of days following infusion (based upon peak incidence of CRS and/or CRES) or until any emergent CRS resolves. Sinus tachycardia can be an early presenting sign of CRS29,95, and recognition of this clinical finding requires high vigilance and awareness of the child’s baseline heart rates as well as age-specific normal values. To enable prompt medical intervention for these toxicities, we recommend that patients have central venous access or a double or triple lumen peripherally inserted central catheter37. For patients with a history of, or predisposition to, seizures or those with a high risk of CNS pseudoprogression (such as those with CNS disease, chloromas and/or leptomeningeal enhancements, or a prior history of seizures), a baseline neurology evaluation, electroencephalography (EEG), and/or baseline MRI of the brain and spinal cord and/or anti-seizure prophylaxis should be considered96.
We recommend that all patients have frequent physical examinations and laboratory monitoring with complete blood counts, comprehensive metabolic panels, coagulation testing, and serum ferritin and C-reactive protein measurements during the post-infusion period associated with a high risk of CRS (as defined in pivotal studies and by emerging data for each product)37,82,84 (Box 1). Regular — daily, if possible — monitoring for TLS is also important, and TLS prophylaxis is recommended for patients with a high disease burden16,29,37,82,84,96. In addition, infectious disease prophylaxis against viral, bacterial, and/or fungal pathogens should be prescribed as appropriate63,82. Patients should have adequate hydration with monitoring for acute fluid overload (daily weights and fluid intake–output recording). If needed, transfusions (irradiated blood products) should be ordered according to institutional guidelines for paediatric patients (without routine corticosteroid pre-medication). The risk of bleeding can be exacerbated by hypofibrinogenaemia and/or thrombocytopenia, especially in patients receiving anti-coagulation therapy (including through continuous venovenous haemofiltration)38,82,97. Conservative management of bleeding or hypofibrinogenaemia and/or thrombocytopenia, with the use of cryoprecipitate or fresh frozen plasma, as needed, is recommended to avoid lethal haemorrhage82. Administration of growth factors (G-CSF) should be considered for patients with neutropenic fever8,82. Conditional orders for fever and/or neutropenia and suspected sepsis can enable rapid intervention, when needed, by the nursing unit, outpatient triage personnel, and pharmacy. We recommend creation of a flag or banner in the electronic health system to alert care providers of patients who are CAR T cell recipients. Indeed, recipients of CAR T cell products should be made easily identifiable; in particular, the strong contraindication of these patients to steroids must be highly visible to avoid routine or accidental administration of these drugs (for example, during blood product and other pre-medication orders). An ‘as needed’ order, which requires a real-time electronic approval by an authorized care-provider, is recommended in order to ensure rapid access to the correct dose of anti-IL-6 therapy (for example, with the anti-IL-6 receptor antibody tocilizumab) when required for the treatment of CRS and/or CRES.
CRS monitoring, grading, and management
CRS reflects a systemic inflammatory response driven by rapid and excessive secretion of cytokines (a so-called cytokine storm) that is associated with a spectrum of symptoms ranging from fever to multi-organ dysfunction34,35. As mentioned previously, 77% of the paediatric and young adult population of the ELIANA trial developed CRS after treatment with tisagenlecleucel, with almost half experiencing severe symptoms requiring intensive-care support38. In addition, 40% of patients developed CRES (grade 3 in 13%)38. Other notable adverse events included infection (in 43% of patients), cytopenias (grade ≥3 neutropenia and thrombocytopenia not resolved by day 28 in 35% and 7%, respectively), and TLS (in 4%)38. Patients with a high risk of developing severe CRS include those with early symptom onset (typically within 3 days of CAR T cell infusion), a high disease burden, and/or pre-existing comorbidities9,20,22.
Early detection of CRS and/or CRES in paediatric patients can be challenging; however, early diagnosis of CRS and its prompt management can mitigate the risks of life-threatening sequelae. We recommend that patients who show signs of CRS and/or CRES be admitted for observation. The grading and management of CRS have been largely based on criteria originally outlined by Lee and colleagues29. In the January 2018 issue of this journal, Neelapu and other members of the MD Anderson Cancer Center CARTOX Program published updated and more-detailed guidelines for the management of adult patients with CRS37. Herein, these recommendations for adult patients have been modified with input from the PALISI Network HSCT Subgroup to provide paediatric-specific guidelines.
CRS grading according to the criteria outlined in Table 2 should be performed at least once every 12 hours and more often if a change in the patient’s clinical status or reasons for concern are noted (Table 1). Parent and/or caregiver concerns should be thoroughly investigated because early signs or symptoms of CRS can be subtle and thus might be best recognized by those who know the child very well98 (Table 1). For example, detection of CRS involving the gastrointestinal system often requires recognition of changes in the child’s food intake and/or the frequency and consistency of bowel movements, as well as expression of nausea. We recommend that CRS grading performed primarily by physicians, advanced-practice providers, and bedside nurses be reviewed by interdisciplinary team members immediately after each assessment and, when possible, should include participation of the patient and/or parent or caregiver at the bedside. Nurses should ideally perform assessments mid-shift and jointly with incoming nurses during handoff at the end of their shifts. In the outpatient setting, properly trained caregivers could potentially perform CRS–CRES assessment in lieu of twice-daily clinical assessments by a health-care professional, but this approach has not been validated.
CRS should be suspected if at least one of the following four symptoms or signs is present during the CRS-risk period after CAR T cell infusion: fever ≥38 °C; hypotension (defined as a systolic blood pressure (SBP) <(70 + (2 × age in years)) mmHg for patients aged 1–10 years or <90 mmHg for those aged >10 years, a change in SBP from baseline values, and/or a reduced requirement for chronic anti-hypertensive medications); hypoxia with an arterial oxygen saturation of <90% on room air; and/or evidence of organ toxicity as determined using the most recent Common Terminology Criteria for Adverse Events version 5.0 (CTCAE v5.0) grading system99 — bearing in mind specific considerations for paediatric patients29,37,82 (Tables 1,2). Frequent monitoring of complete blood count, coagulation, and chemistry profiles, including serum levels of liver enzymes, C-reactive protein, ferritin, and lactate dehydrogenase, might be useful for early detection of CRS.
While the criteria above provide general definitions of hypotension in children, it is important that the baseline blood pressure range of each child be defined before CAR T cell infusion so that relative hypotension from an elevated baseline is not missed. Reduced requirements for chronic anti-hypertensive medications can also indicate relative hypotension. Furthermore, because some symptoms can be caused by other concurrent conditions (for example, sinus tachycardia can have causes that range from crying to sepsis), care providers must use their clinical judgement to determine CRS attribution. Patients who develop fever with a temperature >38 °C, for example, should be assessed for infection using blood cultures and chest radiography; additional tests, such as viral PCR, respiratory viral screening, urine cultures, and CT of the chest, should be obtained as clinically indicated. Empiric antibiotic treatment should be initiated, and filgrastim should be considered if the patient is neutropenic and septic. Importantly, the patient’s orders should ensure triggering of such an escalation in care for a temperature >38 °C — some nursing units might be accustomed to higher temperature thresholds for intervention. This practice might require re-education of outpatient, emergency room, and triage staff that might not routinely care for patients treated with CAR T cells.
Careful vigilance for early recognition of haemodynamic shock in the child is crucial. Symptoms such as malaise, lethargy, weakness, oliguria, irritability, and reduced appetite are not always self-reported by younger children. Among infants, assessment of diapers will be critical to assess urine output and detect diarrhoea. We concur with recommendations that any patient requiring a rapid increase in the dose of vasopressors or exhibiting evidence of end-organ hypoperfusion should be treated intensively for grade 3 CRS, even if the vasopressor therapy required is ‘low dose’ according to the definition of Lee and colleagues29,37. For children with hypotension owing to CRS, an initial normal saline fluid bolus (10–20 ml/kg; maximum 1,000 ml) should be administered; if no improvement is observed, anti-IL-6 therapy should be initiated (Table 2). After administration of anti-IL-6 therapy, the decision to repeat additional fluid boluses versus starting vasopressors should involve consideration of the cardiac and fluid status of the child. For example, the administration of additional fluid boluses should be avoided in patients with underlying cardiac dysfunction and/or signs and symptoms of volume overload (such as pulmonary oedema). Additionally, early use of colloid solutions might be indicated because patients with CRS could potentially develop capillary leak and hypoalbuminaemia more rapidly than patients with sepsis, and the administration of additional fluid boluses might compromise pulmonary function by causing pulmonary oedema. Care should be taken not to trigger acute fluid overload, cardiogenic shock, and/or respiratory compromise. Consideration should be given to adrenal insufficiency in decisions of the initial choice of corticosteroid intervention, if needed (Table 2); patients with vasopressor-resistant hypotension attributed to adrenal insufficiency might respond to stress-dose hydrocortisone only and, thus, high doses of other lymphocytotoxic corticosteroids (dexamethasone or methylprednisolone) can be avoided. Transfer to an intensive-care unit should be considered early in this process (Table 1).
Grading criteria for CRS-related hypoxia have predominantly been based upon fraction of inspired oxygen (FiO2) requirements and the need for mechanical ventilation29,37. In recognition of the differences in acute respiratory distress syndrome (ARDS) between adult and paediatric patients, a 2015 publication by the Pediatric Acute Lung Injury Consensus Conference (PALICC) group provided paediatric-specific definitions for paediatric ARDS (P-ARDS)100; these criteria have since been applied among paediatric patients who have undergone HSCT101,102. We recommend application of the PALICC at-risk P-ARDS criteria for the CRS grading of hypoxia100 (Table 1). Accordingly, the grading of CRS-related hypoxia should be based on the use of high-flow oxygen and other non-invasive forms of mechanical ventilation, whereby flow rates and FiO2 are indicators of severity. For example, paediatric patients who require supplemental oxygen exceeding an FiO2 requirement of 40% or those who are receiving non-invasive mechanical ventilation should be classified as having grade 3 CRS and be managed accordingly (Table 2).
CTCAE v5.0 grading of organ toxicity provides an objective assessment tool, although high vigilance is important for prompt recognition of CRS among children. Sinus tachycardia (according to age-dependent definitions) is often the earliest sign of CRS103 (Table 1). Expected heart rate ranges should also incorporate the child’s baseline measurements at patient admission before CAR T cell infusion104. Acute kidney injury in children can be graded according to CTCAE v5.0 criteria using the Pediatric Risk, Injury, Failure, Loss, End-Stage Renal Disease (pRIFLE) and Kidney Disease: Improving Global Outcomes (KDIGO) definitions of oliguria and anuria105,106 (Tables 1,2).
In one study20, the use of high-dose steroids to treat CRS was associated with suppression of CAR T cell expansion and unfavourable patient outcomes. However, no adequately powered randomized studies investigating whether the administration of anti-IL-6 therapy and/or corticosteroids reduces the efficacy of CAR T cell therapy have been reported to date. Notably, the use of anti-IL-6 therapy and/or corticosteroids for the management of CRS (usually following CAR T cell expansion) has not negatively affected disease-free survival outcomes in larger cohorts107,108. Indeed, the anti-IL-6 receptor antibody tocilizumab is currently approved by the FDA for management of CRS109; patients weighing <30 kg are treated at a dose of 12 mg/kg, and those weighing ≥30 kg are treated at a dose of 8 mg/kg110 (Tables 1,2). Clinical responses following the administration of tocilizumab are often observed within 4 hours (ref.33). Patients who do not respond to the first dose of tocilizumab might be less likely to respond to subsequent repeat administration of this agent; for these patients, consideration should be given to administration of corticosteroids — weighing the risks and benefits of steroid use for CRS and the uncertain risk of suppressing CAR T cell expansion — and/or alternative agents, such as the anti-IL-6 monoclonal antibody siltuximab37,38,111. Corticosteroids can also be administered concurrently with repeat doses of tocilizumab (Table 2). When corticosteroids are used, the taper should be rapid and individualized according to the patient’s response. The initial choice of corticosteroid (hydrocortisone, dexamethasone, or methylprednisolone) will vary depending on institutional preference, protocol-specific guidelines, or product label specifications.
CAR T cell therapy-related HLH
Haemophagocytic lymphohistiocytosis (HLH) is a rare syndrome with severe clinical sequelae that result from a dysregulated, hyperinflammatory immune response112 and can present in a primary (inherited) or a secondary form113. Secondary HLH is thought to occur in the context of an underlying immunological condition and, in the setting of autoimmune and inflammatory disorders, is often referred to as macrophage-activation syndrome (MAS). The diagnosis of HLH is made on the basis of the presence of mutations associated with primary HLH (such as mutations in PRF1, UNC13D, or STX11) and/or clinical and laboratory criteria, such as fever, cytopenias, hypertriglyceridaemia, hypofibrinogenaemia, elevated serum levels of ferritin and liver enzymes, haemophagocytosis, low or absent NK cell activity, and/or elevated soluble IL-2 receptor levels113. Differentiation of primary HLH from MAS can be difficult, and distinction of secondary CAR T cell-related HLH–MAS from CRS–CRES can be even more challenging owing to the overlapping symptoms associated with these conditions16,29,37,112. Future studies to define the role of genetic testing and functional analyses of NK cells might help identify patients who are at a disproportionately higher risk of this complication of CAR T cell therapy30. Nevertheless, diagnostic criteria for this rare toxicity have been provided in the guidelines for the management of adult patients treated with CAR T cell therapy published by Neelapu et al.37 in this journal. Children can also be diagnosed with CAR T cell-related HLH–MAS if they have a peak serum ferritin level >10,000 ng/ml during the CRS-risk period and develop any two of the following: grade ≥3 organ toxicities involving the liver, kidney, or lung; or haemophagocytosis in the bone marrow or other organs37. Patients who develop CAR T cell-related HLH–MAS can be treated simultaneously with anti-IL-6 therapy and corticosteroids (Fig. 1); responses to anti-IL-6 therapy alone might not be as common as in patients with CRS alone33. In addition, although HLH–MAS occurring after treatment with CAR T cells and other T cell-engaging therapies has typically been shown to resolve following administration of anti-IL-6 therapy and/or corticosteroids30,62,96, refractory cases can require additional therapy, including consideration of systemic and/or intrathecal therapy according to the HLH-2004 management guidelines113 or use of the IL-1 receptor antagonist anakinra62,114 (Table 1). Further research is needed, however, to optimize the diagnosis and treatment of CAR T cell-related HLH–MAS.
Neurological symptoms associated with CAR T cell therapy, referred to as CRES, commonly present as a toxic encephalopathy with delirium, seizures, and/or cerebral oedema29,37,62,82,115. The earliest signs and symptoms of CRES can be subtle among children, and, thus, a paediatric skill set is required in order to determine the child’s baseline level of cognitive performance. Among adult patients, early presenting symptoms of CRES include inattention and impaired expression affecting language and handwriting37. Grading of CRES according to CTCAE v5.0 (ref.99) or previously published CRES algorithms for adult patients29,37 is not optimal among infants and younger children. The Cornell Assessment of Pediatric Delirium (CAPD)116 is a validated screening tool (Supplementary Table 2) for recognition of delirium among children and adolescents (from birth to 21 years old); the sensitivity and specificity of this tool are highest in patients aged <12 years. Use of CAPD with appropriate developmental anchor points117 enables developmentally appropriate delirium screening by nurses and other members of the health-care team at the bedside and is an important tool in the overall grading of CRES, as outlined in Table 3; a CAPD score >8 is indicative of delirium116. Alternatively, neurological assessment scoring, as previously described with the CARTOX 10-point assessment scale (CARTOX-10) grading system by Neelapu et al.37, can be used for patients aged ≥12 years who have cognitive abilities that are appropriate for these assessments (Table 3). We recommend that delirium screening with CAPD or other neurological assessments be performed at least twice per day among admitted patients and at least daily among outpatients during the high-risk period for CRES (the 4 weeks after CAR T cell infusion) (Table 1). The first neurological assessment from a nursing provider should occur at the end of their shift and be conducted concurrently with the incoming nurse. The severity of CRES can be labile; thus, assessments should be performed more frequently if a change from prior scores occurs and/or if a caregiver raises concerns. Indeed, the trend in CAPD scores within an individual patient is important; increasing scores can be used as a marker of CRES severity (Table 3).
The onset of CRES can be biphasic, occurring concurrently with CRS and/or after CRS has resolved, and the precise pathophysiology remains unclear — although evidence implicates a combination of endothelial activation in the CNS, elevated cytokine levels in the cerebrospinal fluid (CSF), and cerebral T cell infiltration118,119. The use of anti-IL-6 therapy seems to be more effective for the management of CRES that occurs concurrently with CRS. Patients who develop CRES can also benefit from early corticosteroid administration29,37,38 (Table 3). CRES is generally reversible; however, cerebral oedema and death have been reported37,64,120,121,122. No adequately powered randomized studies to identify patients who are at disproportionately higher risk of CRES have been reported to date. We recommend that recipients of CAR T cell therapy with CNS disease or a history of seizures receive anti-seizure prophylaxis with levetiracetam (10 mg/kg, up to a maximum of 500 mg per dose) every 12 hours for 30 days following infusion (or through the CRES-risk period as described during pivotal studies and with subsequent emerging data)9,27,37 (Box 1). Levetiracetam is generally well-tolerated, with a minimal risk of adverse drug interactions, although dose adjustments might be necessary in the setting of renal dysfunction and are not thought to affect cytokine levels37,123,124. Neurology consultation should be considered if the patient develops grade 1 CRES (Table 3) and/or for specialized screening for papilloedema. Patients should be closely monitored for signs and symptoms of cerebral oedema. Status epilepticus can be managed according to institutional guidelines (our recommended management approaches are provided in Box 4). In general, first-line anti-seizure medications with unfavourable cardiotoxicity profiles (such as lacosamide and phenytoin) should be avoided when possible. Increased intracranial pressure (CSF opening pressure ≥20 mmHg or clinical signs of increased intracranial pressure) will require intensive-care management and osmotherapy (a management algorithm that can be tailored according to institutional guidelines is proposed in Box 5). A neurosurgery consultation should be considered, and brain scans can help guide patient management. Routine chemistry panels should be monitored more frequently (every 6–8 hours) and medications adjusted accordingly to prevent rebound cerebral oedema, renal failure, hypovolemia and/or hypotension, and electrolyte abnormalities. General grading and management guidelines for CRES are outlined in Table 3 (these can be tailored according to product-specific approved label instructions and/or study protocols). CRES can occur as a later complication and, in some patients, after discharge from hospital; therefore, caregivers and/or the patient should be given appropriate anticipatory guidance and appropriate education before being discharged from hospital. Moreover, patients should have a caregiver available who can observe for signs of CRES and seek prompt intervention for at least 4 weeks (or through the CRES-risk period) after CAR T cell infusion.
Long-term follow-up assessment
Careful long-term follow-up assessment of patients receiving CAR T cell therapy is important. Management of on-target, off-tumour effects should be well coordinated between treatment and referring centres if the patient returns to local providers following therapy.
For example, B cell aplasia and hypogammaglobulinaemia or agammaglobulinaemia are commonly seen in patients treated with anti-CD19 CAR T cells. These adverse effects require long-term replacement with intravenous immunoglobulins (IVIGs). We recommend intervention to maintain serum immunoglobulin levels above 400 µg/l with IVIGs as well as consideration of IVIG administration to provide specific immunity during active infection, irrespective of immunoglobulin levels63,125. B cell aplasia has been associated with progressive multifocal leukoencephalopathy (PML)126,127; thus, patients should be closely monitored for neurological signs and symptoms that are suggestive of PML (neuropsychological deficits, progressive dementia, apraxia, or visual and motor deficits)126 until the resolution of B cell aplasia.
Data from studies of the effectiveness and safety of immunization with inactive or live vaccines in patients treated with adoptive T cell therapies have not been reported to date. We recommend careful assessment of immune reconstitution after lymphodepletion and CAR T cell infusion; the findings should guide decisions regarding antimicrobial prophylaxis and re-vaccination63,128.
Patients treated with CAR T cell therapy are at risk of disease relapse and/or the development of secondary neoplasms. In addition, the use of a replication-competent viral vector during CAR manufacturing could pose a theoretical risk to patients and/or their close contacts129. Long-term clinical monitoring is important for detection of these complications and is mandated by the FDA for all such gene therapies130,131.
Local and national registries to capture the outcomes, acute complications, and late effects of CAR T cell therapies might enable the establishment of quality benchmarks, facilitate retrospective research, recognize potential delayed toxicities, and ultimately improve future care. As the Center for International Blood and Marrow Transplant Research (CIBMTR) develops a registry for patients receiving CAR T cell therapy, we recommend that consideration be given to the reporting of variables that are directly retrievable from electronic medical records to ensure accuracy and minimize the infrastructural burden required for comprehensive reporting. Given that toxicity grading systems are likely to evolve over time, entry of primary variables seems more useful in the long term. Furthermore, many of these patients require intensive-care support, and therefore prospective collaborations with intensive-care registries, such as Virtual paediatric intensive-care unit (PICU) Systems (VPS)132, should be considered (Table 1). This approach could enable accurate data entry of cell-therapy variables into the CIBMTR registry by cell-therapy programmes, with concurrent entry of data on intensive-care variables into an appropriate registry by paediatric critical care teams.
CAR T cell therapy as a bridge to HSCT
In ELIANA8, the largest study of CAR T cell therapy involving paediatric patients performed to date, 83% of the infused patients (n = 63) achieved minimal residual disease (MRD)-negative complete remission (CR) or CR with incomplete haematological recovery (CRi). After a median follow-up duration of 4.8 months from response, the median CR and/or CRi duration was not reached (range 1.2 months to >14.1 months). The results of prediction-based modelling suggest that more than half of the patients who received tisagenlecleucel on the ELIANA trial will be alive at 5 years after treatment133. The actual allogeneic HSCT (allo-HSCT) rate among those who achieved a CR or CRi was 12% in the ELIANA trial8.
In another paediatric study46, CD4+ and CD8+ T cells transfected with an anti-CD19 CAR construct containing a 4-1BB co-stimulatory domain using a lentiviral vector were administered to 45 children and young adults with pre-B cell ALL; 93% of the patients achieved MRD-negative remission by day 21. However, the estimated 12-month event-free survival was 50.8%, with the majority of these patients unfortunately experiencing disease relapse46. The persistence of functional anti-CD19 CAR T cells was assessed by measuring the duration of B cell aplasia using flow cytometry; the median duration of B cell aplasia was 3 months (95% CI 2.07–6.44)46. In this study46, 11 of 40 (28%) patients who were in CR underwent allo-HSCT, and 2 of these 11 patients subsequently experienced CD19+ leukaemia relapse.
In an open-label, phase I, dose-escalation study of anti-CD19 CAR T cells (containing a CD28 co-stimulatory domain and manufactured using a retroviral vector) involving children and young adults with ALL or non-Hodgkin lymphoma performed by the US NIH, the CR rate was 66.7%. Following remission, 10 of 12 (83%) patients who achieved MRD-negative remission underwent HSCT and remained disease-free at the time of publication of the data22.
At this time, whether CAR T cell therapy is a definitive treatment remains unclear. While strategies to understand antigen-escape mechanisms and to increase rates of long-term remission are developed134, allo-HSCT can reasonably be considered for patients with haematological malignancies who have achieved remission following CAR T cell therapy. Alternatively, as CAR T cell product-specific data matures, it might also be reasonable to consider CAR T cell therapy as a definitive treatment. The decision to proceed with allo-HSCT should be based upon the candidate meeting standard eligibility requirements, and the long-term outcomes associated with the specific CAR T cell product used should be considered in the risk–benefit assessment.
Currently, CAR T cell therapy for paediatric patients is available for only those with high-grade, relapsed and/or refractory ALL. Remission rates among children with relapsed and/or refractory ALL, who previously had no curative options, have been impressive with current CAR T cell therapies97. Nevertheless, not all children with relapsed and/or refractory ALL are appropriate candidates for this therapy. Patients who do not have a reasonable expectation of survival between leukapheresis and CAR T cell administration or whose survival after CAR T cell therapy is expected to be limited by other comorbidities should not be considered as candidates for this treatment. Among these groups, the risks of primary disease progression must be weighed against the risk of accelerating mortality and/or causing severe disability that could potentially be associated with CAR T cell therapy135.
Financial and health-system considerations
We understand that value in health care is determined by patient outcomes balanced against costs. The current estimated cost of standard of care CAR T cell therapy for children with ALL is high136,137. Moreover, the ancillary administrative and supportive care service (including management of complications, intensive-care unit stays, and frequent hospitalization) costs can be substantially higher than the CAR T cell product price tag138. We strongly encourage consideration of the quality-adjusted life years gained for paediatric patients who can potentially achieve long-term remission as a result of this therapy and encourage all efforts to reduce the costs of care137,139,140 (Table 1). We anticipate that advances in CAR T cell technology will improve our understanding of the pathophysiology of CRS–CRES and facilitate the discovery of predictive biomarkers with which to identify patients requiring early intervention with available supportive care, which will subsequently lead to improved outcomes and reductions in the cost of this care, in addition to biomarkers for identifying in advance patients who are unlikely to respond. As payers and health systems determine coverage benefits, it is important that the specific needs of all children be considered and that access is granted to CAR T cell therapy and the associated supportive care (encompassing baseline assessments, inpatient observation, and essential supportive care when necessary).
Health institutions are encouraged to provide access to CAR T cell therapies; however, adequate strategic and operational planning and preparation are needed to ensure the safe delivery of such treatments. We recommend that programmes seek immune effector cell (IEC) accreditation by the FACT as a voluntary means of ensuring adherence to quality standards55 (Table 1). FACT accreditation will require an established quality-assurance programme at the institution, as well as education and ongoing training for clinical staff. Institutions that offer CAR T cell therapy should support rigorous quality assurance, data management, clinical services, and education programmes for interdisciplinary staff involved in the care of patients who receive this treatment. Additionally, emergency medical services, community hospitals, and local triage facilities will require high vigilance to recognize and promptly escalate care in the event that a patient treated with CAR T cell therapy presents to their facility in an emergency.
The availability of skilled interdisciplinary staff, including nurses, is an essential requirement for safe administration of CAR T cell therapy to paediatric patients. Owing to the need for close medical attention among these patients, communication between coordinators, medical care providers, and nursing administration is important to ensuring that CAR T cell infusions are considered together with staffing decisions141,142. Inpatient and outpatient nursing units, triage facilities, emergency-care departments, and intensive-care units all need available nursing staff that have completed all required competencies to care for patients treated with CAR T cells. These nurses can assist in the rapid recognition of CRS–CRES and help avoid iatrogenic errors (for example, administration of steroids as a routine pre-medication). Visual cues, such as flags in the patient chart or patient bracelets (similar to ‘fall risk’ identifiers), can help care teams to quickly recognize recipients of CAR T cells, even during electronic medical record ‘downtime procedures’. Discharge protocols should ensure comprehensive education of the caregiver and patient about signs and symptoms of CRS and CRES, and the patient should also be given a wallet or REMS identification card for their specific CAR T cell product. Most importantly, recipients of CAR T cells should be instructed to immediately alert all providers that they have received this therapy, especially if presenting to a facility outside of their original treatment centre.
As CAR T cell therapies transition from experimental therapies to standard-of-care treatments, institutional pharmacists must be engaged in the development of policies and protocols to optimize supportive care. Additionally, REMS programmes are likely to accompany new therapy approvals by the FDA. For instance, the REMS programme for tisagenlecleucel requires institutions to have a minimum of two doses of tocilizumab available on site for each patient at risk of CRS–CRES in order to enable immediate administration143. New CAR T cell therapies might also require an adequate stock of other agents directed at ameliorating CRS and/or CRES and other supportive therapies (such as rituximab and cetuximab for the aforementioned products with integrated suicide or safety switches)49,56,61. Institutional pharmacies will need to ensure adequate training of pharmacy staff regarding the recognition and management of CRS and CRES and the development of policies and protocols to ensure adequate stock and the prompt dispensation of supportive therapies55.
Emergency contingency planning
Institutions involved in the administration of CAR T cell therapies are encouraged to develop programme-specific emergency plans that take into account the particular needs of patients exposed to these treatments144. Many patients will be required to live within a specified distance of the treating hospital for a predefined amount of time after CAR T cell infusion. Should acute toxicities develop, this stipulation will ensure prompt access to care, which will help to mitigate against additional complications. When possible, institutions should develop protocols to overcome barriers to care for these patients in the event of natural disasters and/or disruption of services. Prespecified electronic medical record downtime procedures, evacuation plans that include access to anti-IL-6 therapies, and the consideration of admission of outpatients at risk of CRS–CRES before an anticipated natural disaster are examples of disaster planning protocols145,146.
To achieve improvements in CAR T cell therapy and the requisite supportive care, and thereby sustain CR rates among children, we recommend that enrolment of children in trials of novel agents be facilitated at the earliest acceptable time points. In this regard, application of CAR T cell therapies for treatment of solid tumours and other haematological malignancies in children is being explored (NCT03126864, NCT01953900, NCT02311621, and NCT03056339). Moreover, consideration of earlier or upfront use of CAR T cell therapy might spare patients the acute and long-term toxicities associated with traditional chemotherapy and/or radiation regimens. Whether CAR T cell therapy should be followed by allo-HSCT or repeat CAR T cell infusions also needs to be explored. Future studies aimed at improving the persistence of CAR T cells and thereby inducing long-term remission without the need for further therapy are an important requirement. An overarching commitment to improve patient outcomes, especially among children with limited or no therapeutic options, is key.
Currently, the time required from leukapheresis to manufacture CAR-expressing cells is a major limitation of CAR T cell therapy. Efforts to explore third-party, off-the-shelf allogeneic approaches to therapy with CAR T cells or other immune effectors, such as CAR NK cells, are exciting potential alternatives60.
Additional long-term prospective studies to understand the pathophysiology and develop early recognition and optimal treatment strategies for CRS and/or CRES are needed. Such studies should involve broad collaborative efforts because these toxicities are multifaceted and necessitate interdisciplinary care. Response and adverse event rates, as well as late effects, are likely to vary depending on various host, disease, and CAR T cell characteristics; therefore, a comprehensive and robust registry is needed to guide future efforts to optimize and expand the use of CAR T cell therapy.
Noone, A. M. et al. SEER Cancer Statistics Review, 1975–2015. National Cancer Institute https://seer.cancer.gov/csr/1975_2015/ (2018).
Farber, S. & Diamond, L. K. Temporary remissions in acute leukemia in children produced by folic acid antagonist, 4-aminopteroyl-glutamic acid. N. Engl. J. Med. 238, 787–793 (1948).
O’Leary, M., Krailo, M., Anderson, J. R. Reaman, G. H., & Children’s Oncology Group. Progress in childhood cancer: 50 years of research collaboration, a report from the Children’s Oncology Group. Semin. Oncol. 35, 484–493 (2008).
Blau, C. A. E. Donnall Thomas, M.D. (1920–2012). Stem Cells Transl Med. (2013).
Ko, R. H. et al. Outcome of patients treated for relapsed or refractory acute lymphoblastic leukemia: a Therapeutic Advances in Childhood Leukemia Consortium study. J. Clin. Oncol. 28, 648–654 (2010).
Sun, W. et al. Outcome of children with multiply relapsed B cell acute lymphoblastic leukemia: a therapeutic advances in childhood leukemia and lymphoma study. Leukemia https://doi.org/10.1038/s41375-018-0094-0 (2018).
Thomas, E. D. et al. Marrow transplantation for patients with acute lymphoblastic leukemia in remission. Blood 54, 468–476 (1979).
Novartis Pharmaceuticals Corporation. Package insert - KymriahTM (tisagenlecleucel). Novartis Pharmaceuticals Corporation https://www.fda.gov/downloads/BiologicsBloodVaccines/CellularGeneTherapyProducts/ApprovedProducts/UCM573941.pdf (2018).
Maude, S. L. et al. Chimeric antigen receptor T cells for sustained remissions in leukemia. N. Engl. J. Med. 371, 1507–1517 (2014).
Dai, H., Wang, Y., Lu, X. & Han, W. Chimeric antigen receptors modified T-cells for cancer therapy. J. Natl Cancer Inst. 108, djv439 (2016).
Kebriaei, P. et al. Phase I trials using Sleeping Beauty to generate CD19-specific CAR T cells. J. Clin. Invest. 126, 3363–3376 (2016).
Abate-Daga, D. & Davila, M. L. CAR models: next-generation CAR modifications for enhanced T cell function. Mol. Ther. Oncolyt. 3, 16014 (2016).
Maus, M. V. & Levine, B. L. Chimeric antigen receptor T-cell therapy for the community oncologist. Oncologist 21, 608–617 (2016).
Kochenderfer, J. N. et al. Eradication of B-lineage cells and regression of lymphoma in a patient treated with autologous T cells genetically engineered to recognize CD19. Blood 116, 4099–4102 (2010).
Porter, D. L., Levine, B. L., Kalos, M., Bagg, A. & June, C. H. Chimeric antigen receptor-modified T cells in chronic lymphoid leukemia. N. Engl. J. Med. 365, 725–733 (2011).
Grupp, S. A. et al. Chimeric antigen receptor-modified T cells for acute lymphoid leukemia. N. Engl. J. Med. 368, 1509–1518 (2013).
Brentjens, R. J. et al. CD19-targeted T cells rapidly induce molecular remissions in adults with chemotherapy-refractory acute lymphoblastic leukemia. Sci. Transl Med. 5, 177ra138 (2013).
Cruz, C. R. et al. Infusion of donor-derived CD19-redirected virus-specific T cells for B cell malignancies relapsed after allogeneic stem cell transplant: a phase 1 study. Blood 122, 2965–2973 (2013).
Kochenderfer, J. N. et al. Donor-derived CD19-targeted T cells cause regression of malignancy persisting after allogeneic hematopoietic stem cell transplantation. Blood 122, 4129–4139 (2013).
Davila, M. L. et al. Efficacy and toxicity management of 19-28z CAR T cell therapy in B cell acute lymphoblastic leukemia. Sci. Transl Med. 6, 224ra225 (2014).
Kochenderfer, J. N. et al. Chemotherapy-refractory diffuse large B cell lymphoma and indolent B cell malignancies can be effectively treated with autologous T cells expressing an anti-CD19 chimeric antigen receptor. J. Clin. Oncol. 33, 540–549 (2015).
Lee, D. W. et al. T cells expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults: a phase 1 dose-escalation trial. Lancet 385, 517–528 (2015).
Garfall, A. L. et al. Chimeric antigen receptor T cells against CD19 for multiple myeloma. N. Engl. J. Med. 373, 1040–1047 (2015).
Brudno, J. N. et al. Allogeneic T cells that express an anti-CD19 chimeric antigen receptor induce remissions of B-cell malignancies that progress after allogeneic hematopoietic stem-cell transplantation without causing graft-versus-host disease. J. Clin. Oncol. 34, 1112–1121 (2016).
Turtle, C. J. et al. Durable molecular remissions in chronic lymphocytic leukemia treated with CD19-specific chimeric antigen receptor-modified T cells after failure of ibrutinib. J. Clin. Oncol. 35, 3010–3020 (2017).
Locke, F. L. et al. Phase 1 results of ZUMA-1: a multicenter study of KTE-C19 anti-CD19 CAR T cell therapy in refractory aggressive lymphoma. Mol. Ther. 25, 285–295 (2017).
Neelapu, S. S. et al. Axicabtagene ciloleucel CAR T-cell therapy in refractory large B-cell lymphoma. N. Engl. J. Med. 377, 2531–2544 (2017).
Kochenderfer, J. N. et al. B cell depletion and remissions of malignancy along with cytokine-associated toxicity in a clinical trial of anti-CD19 chimeric-antigen-receptor-transduced T cells. Blood 119, 2709–2720 (2012).
Lee, D. W. et al. Current concepts in the diagnosis and management of cytokine release syndrome. Blood 124, 188–195 (2014).
Teachey, D. T. et al. Cytokine release syndrome after blinatumomab treatment related to abnormal macrophage activation and ameliorated with cytokine-directed therapy. Blood 121, 5154–5157 (2013).
Rezvani, K. & Rouce, R. H. The application of natural killer cell immunotherapy for the treatment of cancer. Front. Immunol. 6, 578 (2015).
Richman, S. A. et al. High-affinity GD2-specific CAR T cells induce fatal encephalitis in a preclinical neuroblastoma model. Cancer Immunol. Res. 6, 36–46 (2018).
Fitzgerald, J. C. et al. Cytokine release syndrome after chimeric antigen receptor T cell therapy for acute lymphoblastic leukemia. Crit. Care Med. 45, e124–e131 (2017).
Norelli, M. et al. Monocyte-derived IL-1 and IL-6 are differentially required for cytokine-release syndrome and neurotoxicity due to CAR T cells. Nat. Med. 24, 739–748 (2018).
Giavridis, T. et al. CAR T cell-induced cytokine release syndrome is mediated by macrophages and abated by IL-1 blockade. Nat. Med. 24, 731–738 (2018).
FDA. FDA briefing document: Oncologic Drugs Advisory Committee meeting; BLA 125646; Tisagenlecleucel, Novartis Pharmaceuticals Corporation. FDA https://www.fda.gov/downloads/AdvisoryCommittees/CommitteesMeetingMaterials/Drugs/OncologicDrugsAdvisoryCommittee/UCM566166.pdf (2017).
Neelapu, S. S. et al. Chimeric antigen receptor T cell therapy - assessment and management of toxicities. Nat. Rev. Clin. Oncol. 15, 47–62 (2018).
Maude, S. L. et al. Tisagenlecleucel in children and young adults with B-cell lymphoblastic leukemia. N. Engl. J. Med. 378, 439–448 (2018).
Fink, A., Kosecoff, J., Chassin, M. & Brook, R. H. Consensus methods: characteristics and guidelines for use. Am. J. Publ. Health 74, 979–983 (1984).
Shekelle, P. G., Woolf, S. H., Eccles, M. & Grimshaw, J. Developing clinical guidelines. West J. Med. 170, 348–351 (1999).
Shields, A. F. et al. Immune modulation therapy and imaging: workshop report. J. Nucl. Med. 59, 410–417 (2018).
Massad, N., Lee, S. C., Lasala, P. A. & Welch, M. R. A case of pembrolizumab-induced central nervous system toxicity in a patient with metastatic melanoma. Am. Acad. Neurol. 86, (Suppl. 16), P4.238 (2016).
Hochmair, M. J., Schwab, S., Burghuber, O. C., Krenbek, D. & Prosch, H. Symptomatic pseudo-progression followed by significant treatment response in two lung cancer patients treated with immunotherapy. Lung Cancer 113, 4–6 (2017).
[No authors listed.] Informed consent, parental permission, and assent in pediatric practice. Committee on Bioethics, American Academy of Pediatrics. Pediatrics 95, 314–317 (1995).
McGuirk, J. et al. Building blocks for institutional preparation of CTL019 delivery. Cytotherapy 19, 1015–1024 (2017).
Gardner, R. A. et al. Intent-to-treat leukemia remission by CD19 CAR T cells of defined formulation and dose in children and young adults. Blood 129, 3322–3331 (2017).
Crookston, K. P. & Simon, T. L. in Apheresis: Principles and Practice 2nd edn (eds McLeod, B. C., Price, T. H. & Weinstein, R.) 71–90 (AABB Press, Bethesda, 2003).
Goldstein, S. L. Therapeutic apheresis in children: special considerations. Semin. Dial 25, 165–170 (2012).
US National Library of Medicine. ClinicalTrials.gov https://clinicaltrials.gov/ct2/show/NCT02028455 (2018).
Michon, B. et al. Complications of apheresis in children. Transfusion 47, 1837–1842 (2007).
Gorlin, J. B. et al. Pediatric large volume peripheral blood progenitor cell collections from patients under 25 kg: a primer. J. Clin. Apheresis 11, 195–203 (1996).
Carausu, L., Clapisson, G., Philip, I., Sebban, H. & Marec-Berard, P. Use of totally implantable catheter for peripheral blood stem cell apheresis. Bone Marrow Transpl. 39, S131–S131 (2007).
Koristek, Z., Sterba, J., Havranova, D. & Mayer, J. Technique for PBSC harvesting in children of weight under 10 kg. Bone Marrow Transpl. 29, 57–61 (2002).
Ohara, Y. et al. Comprehensive technical and patient-care optimization in the management of pediatric apheresis for peripheral blood stem cell harvesting. Transfus Apher Sci. 55, 338–343 (2016).
Foundation for the Accreditation of Cellular Therapy. FACT Standards for Immune Effector Cells. 1st edn (FACT, 2017).
Wells, J. et al. Pre-clinical activity of allogeneic anti-CD22 CAR-T cells for the treatment of B-cell acute lymphoblastic leukemia. Blood 130, 808 (2017).
US National Library of Medicine. ClinicalTrials.gov https://clinicaltrials.gov/ct2/show/NCT03056339 (2018).
US National Library of Medicine. ClinicalTrials.gov https://clinicaltrials.gov/ct2/show/NCT02808442 (2018).
Rezvani, K., Rouce, R., Liu, E. & Shpall, E. Engineering natural killer cells for cancer immunotherapy. Mol. Ther. 25, 1769–1781 (2017).
Liu, E. et al. Cord blood NK cells engineered to express IL-15 and a CD19-targeted CAR show long-term persistence and potent antitumor activity. Leukemia 32, 520–531 (2017).
Philip, B. RQR8: A universal safety switch for cellular therapies. Thesis, Univ. College London (2015).
Maude, S. L., Barrett, D., Teachey, D. T. & Grupp, S. A. Managing cytokine release syndrome associated with novel T cell-engaging therapies. Cancer J. 20, 119–122 (2014).
Tomblyn, M. et al. Guidelines for preventing infectious complications among hematopoietic cell transplantation recipients: a global perspective. Biol. Blood Marrow Transplant 15, 1143–1238 (2009).
Turtle, C. J. et al. CD19 CAR-T cells of defined CD4(+): CD8(+) composition in adult B cell ALL patients. J. Clin. Invest. 126, 2123–2138 (2016).
Dudley, M. E. et al. Adoptive cell transfer therapy following non-myeloablative but lymphodepleting chemotherapy for the treatment of patients with refractory metastatic melanoma. J. Clin. Oncol. 23, 2346–2357 (2005).
Dummer, W. et al. T cell homeostatic proliferation elicits effective antitumor autoimmunity. J. Clin. Invest. 110, 185–192 (2002).
Klebanoff, C. A., Khong, H. T., Antony, P. A., Palmer, D. C. & Restifo, N. P. Sinks, suppressors and antigen presenters: how lymphodepletion enhances T cell-mediated tumor immunotherapy. Trends Immunol. 26, 111–117 (2005).
Mackall, C. L., Hakim, F. T. & Gress, R. E. Restoration of T cell homeostasis after T cell depletion. Semin. Immunol. 9, 339–346 (1997).
Tanchot, C., Lemonnier, F. A., Perarnau, B., Freitas, A. A. & Rocha, B. Differential requirements for survival and proliferation of CD8 naive or memory T cells. Science 276, 2057–2062 (1997).
Goldrath, A. W. & Bevan, M. J. Low-affinity ligands for the TCR drive proliferation of mature CD8+ T cells in lymphopenic hosts. Immunity 11, 183–190 (1999).
Tan, J. T. et al. Interleukin (IL)-15 and IL-7 jointly regulate homeostatic proliferation of memory phenotype CD8+ cells but are not required for memory phenotype CD4+ cells. J. Exp. Med. 195, 1523–1532 (2002).
Schluns, K. S., Kieper, W. C., Jameson, S. C. & Lefrancois, L. Interleukin-7 mediates the homeostasis of naive and memory CD8 T cells in vivo. Nat. Immunol. 1, 426–432 (2000).
Tan, J. T. et al. IL-7 is critical for homeostatic proliferation and survival of naive T cells. Proc. Natl Acad. Sci. USA 98, 8732–8737 (2001).
Shimizu, J., Yamazaki, S. & Sakaguchi, S. Induction of tumor immunity by removing CD25+CD4+ T cells: a common basis between tumor immunity and autoimmunity. J. Immunol. 163, 5211–5218 (1999).
Colombo, M. P. & Piconese, S. Regulatory-T cell inhibition versus depletion: the right choice in cancer immunotherapy. Nat. Rev. Cancer 7, 880–887 (2007).
Wrzesinski, C. et al. Increased intensity lymphodepletion enhances tumor treatment efficacy of adoptively transferred tumor-specific T cells. J. Immunother. 33, 1–7 (2010).
Gattinoni, L., Powell, D. J. Jr., Rosenberg, S. A. & Restifo, N. P. Adoptive immunotherapy for cancer: building on success. Nat. Rev. Immunol. 6, 383–393 (2006).
Cameron, J. et al. Addition of fludarabine to cyclophosphamide lymphodepletion improves in vivo expansion of CD19 chimeric antigen receptor-modified T cells and clinical outcome in adults with B cell acute lymphoblastic leukemia. Blood 126, 3773 (2015).
Heczey, A. et al. CAR T cells administered in combination with lymphodepletion and PD-1 inhibition to patients with neuroblastoma. Mol. Ther. 25, 2214–2224 (2017).
Shank, B. R. et al. Chimeric antigen receptor T cells in hematologic malignancies. Pharmacotherapy 37, 334–345 (2017).
Brentjens, R. J. et al. Safety and persistence of adoptively transferred autologous CD19-targeted T cells in patients with relapsed or chemotherapy refractory B cell leukemias. Blood 118, 4817–4828 (2011).
Brudno, J. N. & Kochenderfer, J. N. Toxicities of chimeric antigen receptor T cells: recognition and management. Blood 127, 3321–3330 (2016).
US National Library of Medicine. ClinicalTrials.gov https://clinicaltrials.gov/ct2/show/NCT02203825 (2018).
Leukemia & Lymphoma Society. Chimeric antigen receptor (CAR) T-cell therapy. Leukemia & Lymphoma Society https://www.lls.org/sites/default/files/National/USA/Pdf/Publications/FSHP1_CART_Factsheet_June2018_FINAL.pdf (2017).
Brentjens, R., Yeh, R., Bernal, Y., Riviere, I. & Sadelain, M. Treatment of chronic lymphocytic leukemia with genetically targeted autologous T cells: case report of an unforeseen adverse event in a phase I clinical trial. Mol. Ther. 18, 666–668 (2010).
Truong, T. H. et al. Adverse reactions during stem cell infusion in children treated with autologous and allogeneic stem cell transplantation. Bone Marrow Transplant 51, 680–686 (2016).
Davis, J. M., Rowley, S. D., Braine, H. G., Piantadosi, S. & Santos, G. W. Clinical toxicity of cryopreserved bone marrow graft infusion. Blood 75, 781–786 (1990).
Stroncek, D. F. et al. Adverse reactions in patients transfused with cryopreserved marrow. Transfusion 31, 521–526 (1991).
Zambelli, A. et al. Clinical toxicity of cryopreserved circulating progenitor cells infusion. Anticancer Res. 18, 4705–4708 (1998).
Zenhausern, R., Tobler, A., Leoncini, L., Hess, O. M. & Ferrari, P. Fatal cardiac arrhythmia after infusion of dimethyl sulfoxide-cryopreserved hematopoietic stem cells in a patient with severe primary cardiac amyloidosis and end-stage renal failure. Ann. Hematol. 79, 523–526 (2000).
Hoyt, R., Szer, J. & Grigg, A. Neurological events associated with the infusion of cryopreserved bone marrow and/or peripheral blood progenitor cells. Bone Marrow Transplant 25, 1285–1287 (2000).
Otrock, Z. K. et al. Transient global amnesia associated with the infusion of DMSO-cryopreserved autologous peripheral blood stem cells. Haematologica 93, e36–e37 (2008).
Miniero, R., Vai, S., Giacchino, M., Giubellino, C. & Madon, E. Severe respiratory depression after autologous bone marrow infusion. Haematologica 77, 98–99 (1992).
Shu, Z., Heimfeld, S. & Gao, D. Hematopoietic stem cell transplantation with cryopreserved grafts: adverse reactions after transplantation and cryoprotectant removal prior to infusion. Bone Marrow Transpl. 49, 469–476 (2014).
Breslin, S. Cytokine-release syndrome: overview and nursing implications. Clin. J. Oncol. Nurs. 11, 37–42 (2007).
Namuduri, M. & Brentjens, R. J. Medical management of side effects related to CAR T cell therapy in hematologic malignancies. Expert Rev. Hematol. 9, 511–513 (2016).
Buechner, J. et al. Global registration trial of efficacy and safety of CTL019 in pediatric and young adult patients with relapsed/refractory (r/r) acute lymphoblastic leukemia (ALL): update to the interim analysis. Clin. Lymphoma Myeloma Leuk. 17, S263–S264 (2017).
Power, N. & Franck, L. Parent participation in the care of hospitalized children: a systematic review. J. Adv. Nurs. 62, 622–641 (2008).
U.S. Department of Health and Human Services. Common Terminology Criteria for Adverse Events (CTCAE) v5.0. U.S. Department of Health and Human Services. https://ctep.cancer.gov/protocoldevelopment/electronic_applications/docs/CTCAE_v5_Quick_Reference_5x7.pdf (2017).
Pediatric Acute Lung Injury Consensus Conference Group. Pediatric acute respiratory distress syndrome: consensus recommendations from the Pediatric Acute Lung Injury Consensus Conference. Pediatr. Crit. Care Med. 16, 428–439 (2015).
Rowan, C. M. et al. Pediatric acute respiratory distress syndrome in pediatric allogeneic hematopoietic stem cell transplants: a multicenter study. Pediatr. Crit. Care Med. 18, 304–309 (2017).
Rowan, C. M. et al. High-frequency oscillatory ventilation use and severe pediatric ARDS in the pediatric hematopoietic cell transplant recipient. Respir. Care 63, 404–411 (2017).
Chong, S. L. et al. A retrospective review of vital signs and clinical outcomes of febrile infants younger than 3 months old presenting to the emergency department. PLOS One 13, e0190649 (2018).
Fleming, S. et al. Normal ranges of heart rate and respiratory rate in children from birth to 18 years of age: a systematic review of observational studies. Lancet 377, 1011–1018 (2011).
Akcan-Arikan, A. et al. Modified RIFLE criteria in critically ill children with acute kidney injury. Kidney Int. 71, 1028–1035 (2007).
Kidney Disease Improving Global Outcomes. KDIGO Clinical Practice Guideline for Acute Kidney Injury. Kidney Int. Suppl. 2, 1–138 (2012).
Neelapu, S. S. et al. Axicabtagene ciloleucel (AXI-CEL; KTE-C19) in patients with refractory aggressive non-hodgkin lymphomas (NHL): primary results of the pivotal trial ZUMA-1. Hematol.Oncol. 35 (Suppl.), 28 (2017).
Thudium Mueller, K. et al. CTL019 clinical pharmacology and biopharmaceutics in pediatric patients (pts) with relapsed or refractory (r/r) acute lymphoblastic leukemia (ALL) [abstract ALL-146]. Clin. Lymphoma Myeloma Leuk. 17 (Suppl. 2), 217–218 (2017).
FDA. FDA approves tisagenlecleucel for B cell ALL and tocilizumab for cytokine release syndrome. FDA https://www.fda.gov/drugs/informationondrugs/approveddrugs/ucm574154.htm (2017).
Genentech. ACTEMRA® (tocilizumab) prescribing information. FDA https://www.accessdata.fda.gov/drugsatfda_docs/label/2017/125276s114lbl.pdf (2017).
Chen, F. et al. Measuring IL-6 and sIL-6R in serum from patients treated with tocilizumab and/or siltuximab following CAR T cell therapy. J. Immunol. Methods 434, 1–8 (2016).
Minoia, F. et al. Development and initial validation of the macrophage activation syndrome/primary hemophagocytic lymphohistiocytosis score, a diagnostic tool that differentiates primary hemophagocytic lymphohistiocytosis from macrophage activation syndrome. J. Pediatr. 189, 72–78 (2017).
Henter, J. I. et al. HLH-2004: diagnostic and therapeutic guidelines for hemophagocytic lymphohistiocytosis. Pediatr. Blood Cancer 48, 124–131 (2007).
Frey, N. V. et al. Refractory cytokine release syndrome in recipients of chimeric antigen receptor (CAR) T cells. Blood 124, 2296 (2014).
Hu, Y. et al. Predominant cerebral cytokine release syndrome in CD19-directed chimeric antigen receptor-modified T cell therapy. J. Hematol. Oncol. 9, 70 (2016).
Traube, C. et al. Cornell assessment of pediatric delirium: a valid, rapid, observational tool for screening delirium in the PICU. Crit. Care Med. 42, 656–663 (2014).
Silver, G., Kearney, J., Traube, C. & Hertzig, M. Delirium screening anchored in child development: the Cornell Assessment for Pediatric Delirium. Palliat. Support. Care 13, 1005–1011 (2015).
Gust, J. et al. Endothelial activation and blood-brain barrier disruption in neurotoxicity after adoptive immunotherapy with CD19 CAR-T cells. Cancer Discov. 7, 1404–1419 (2017).
Taraseviciute, A. et al. Chimeric antigen receptor T cell-mediated neurotoxicity in non-human primates. Cancer Discov. 8, 750–763 (2018).
Turtle, C. J. et al. Immunotherapy of non-Hodgkin’s lymphoma with a defined ratio of CD8+ and CD4+ CD19-specific chimeric antigen receptor-modified T cells. Sci. Transl Med. 8, 355ra116 (2016).
Teachey, D. T. et al. Identification of predictive biomarkers for cytokine release syndrome after chimeric antigen receptor T cell therapy for acute lymphoblastic leukemia. Cancer Discov. 6, 664–679 (2016).
Stephen, J. et al. Sustained remissions following chimeric antigen receptor modified T cells directed against CD19 (CTL019) in patients with relapsed or refractory CD19+ lymphomas. Blood 126, 183–183 (2015).
Hovinga, C. A. Levetiracetam: a novel antiepileptic drug. Pharmacotherapy 21, 1375–1388 (2001).
Guenther, S. et al. Chronic valproate or levetiracetam treatment does not influence cytokine levels in humans. Seizure 23, 666–669 (2014).
Perez, E. E. et al. Update on the use of immunoglobulin in human disease: a review of evidence. J. Allergy Clin. Immunol. 139, S1–S46 (2017).
Weissert, R. Progressive multifocal leukoencephalopathy. J. Neuroimmunol. 231, 73–77 (2011).
Durali, D., de Goer de Herve, M. G., Gasnault, J. & Taoufik, Y. B cells and progressive multifocal leukoencephalopathy: search for the missing link. Front. Immunol. 6, 241 (2015).
Abdel-Azim, H., Elshoury, A., Mahadeo, K. M., Parkman, R. & Kapoor, N. Humoral immune reconstitution kinetics after allogeneic hematopoietic stem cell transplantation in children: a maturation block of IgM memory B cells may lead to impaired antibody immune reconstitution. Biol. Blood Marrow Transplant 23, 1437–1446 (2017).
Dalba, C., Bellier, B., Kasahara, N. & Klatzmann, D. Replication-competent vectors and empty virus-like particles: new retroviral vector designs for cancer gene therapy or vaccines. Mol. Ther. 15, 457–466 (2007).
Centre for Biologics Evaluation and Research. Gene therapy clinical trials–observing subjects for delayed adverse events. FDA https://www.fda.gov/downloads/BiologicsBloodVaccines/GuidanceComplianceRegulatoryInformation/Guidances/CellularandGeneTherapy/ucm078719.pdf (2006).
Centre for Biologics Evaluation and Research. Supplemental guidance on testing for replication competent retrovirus in retroviral vector based gene therapy products and during follow-up of patients in clinical trials using retroviral vectors. FDA https://www.fda.gov/downloads/biologicsbloodvaccines/guidancecomplianceregulatoryinformation/guidances/cellularandgenetherapy/ucm078723.pdf (2006).
Bennett, T. D. et al. Existing data analysis in pediatric critical care research. Front. Pediatr. 2, 79 (2014).
Cope, S. et al. Expert elicitation of long-term survival for pediatric acute lymphoblastic leukemia patients receiving CTL019 in Eliana phase II study. Blood 130, 3377 (2017).
Jackson, H. J. & Brentjens, R. J. Overcoming antigen escape with CAR T cell therapy. Cancer Discov. 5, 1238–1240 (2015).
Doorenbos, A. et al. Palliative care in the pediatric ICU: challenges and opportunities for family-centered practice. J. Soc. Work End Life Palliat Care 8, 297–315 (2012).
Yanni Hao, L. K. Cost-effectiveness analysis of CTL019 for the treatment of pediatric and young adult patients with relapsed or refractory B-cell acute lymphoblastic leukemia in the United States. Blood 130, 609 (2017).
LaMattina, J. Pharma’s paradox: cure a deadly childhood disease and then get attacked on price. Forbes https://www.forbes.com/sites/johnlamattina/2017/11/28/cure-a-deadly-childhood-disease-and-then-get-attacked-on-price/#7089d1696479 (2017).
Prasad, V. Immunotherapy: tisagenlecleucel - the first approved CAR-T cell therapy: implications for payers and policy makers. Nat. Rev. Clin. Oncol. 15, 11–12 (2018).
Sanders, G. D. et al. Recommendations for conduct, methodological practices, and reporting of cost-effectiveness analyses: second panel on cost-effectiveness in health and medicine. JAMA 316, 1093–1103 (2016).
Gold, M. Panel on cost-effectiveness in health and medicine. Med. Care 34, DS197–199 (1996).
American Nurses Association. Care coordination and nurses’ essential role. Am. Nurses Associ. https://www.nursingworld.org/~4afbf2/globalassets/practiceandpolicy/health-policy/cnpe-care-coord-position-statement-final--draft-6-12-2012.pdf (2012).
Antonelli, R. C., Stille, C. J. & Antonelli, D. M. Care coordination for children and youth with special health care needs: a descriptive, multisite study of activities, personnel costs, and outcomes. Pediatrics 122, e209–e216 (2008).
FDA. Approved Risk Evaluation and Mitigation Strategies (REMS) for KymriahTM(tisagenlecleucel). FDA https://www.Accessdata.fda.gov/Scripts/Cder/Rems/Index.cfm?Event=IndvRemsDetails.Page&REMS=368 (2017).
Bayntun, C., Rockenschaub, G. & Murray, V. Developing a health system approach to disaster management: A qualitative analysis of the core literature to complement the WHO Toolkit for assessing health-system capacity for crisis management. PLOS Curr. 4, e5028b6037259a (2012).
Kevin, H., Morchel, H., Raheem, M. & Stevens, L. Electronic health records access during a disaster. Online J. Publ. Health Inform 5, 232 (2014).
Wingard, J. R. et al. Preparing for the unthinkable: emergency preparedness for the hematopoietic cell transplant program. Biol. Blood Marrow Transplant 12, 1229–1238 (2006).
Frisen, L. Swelling of the optic nerve head: a staging scheme. J. Neurol. Neurosurg. Psychiatry 45, 13–18 (1982).
The authors thank N. Shah (US National Cancer Institute) and the Evidence-based Practice Committee of the American Pediatric Hematology Oncology Nursing (APHON) Association for their critical review of this manuscript. The authors also acknowledge their patients and their families and all interdisciplinary staff members who participate in their care. Finally, the authors acknowledge collaborative researchers who have provided guidance in the development of these guidelines.
Nature Reviews Clinical Oncology thanks the four anonymous reviewers for their contribution to the peer review of this work.
H.A.-A. has a patent with applications in the field of gene-modified T cell therapy for cancer. C.M.B. has served on the scientific advisory boards of Cellectis, NexImmune, and Torque Pharma. S.S.N. has received research support from Cellectis, Kite Pharma (a Gilead company), and Poseida Therapeutics and is an advisory board member or consultant for Celgene, Kite Pharma, Novartis, and Unum Therapeutics. All other authors declare no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Mahadeo, K.M., Khazal, S.J., Abdel-Azim, H. et al. Management guidelines for paediatric patients receiving chimeric antigen receptor T cell therapy. Nat Rev Clin Oncol 16, 45–63 (2019). https://doi.org/10.1038/s41571-018-0075-2
Chimeric antigen receptor- and natural killer cell receptor-engineered innate killer cells in cancer immunotherapy
Cellular & Molecular Immunology (2021)
Cellular & Molecular Immunology (2021)
Nature Reviews Clinical Oncology (2021)
Nature Reviews Immunology (2021)
Cellular Oncology (2021)