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Tiny but bright

Ten years ago, the engineering of a small luciferase called NanoLuc broke through a common limitation of bioluminescence. Currently the brightest known bioluminescent protein, NanoLuc’s activity has been used across a huge application range — enabling measurements in single cells and in whole living organisms.

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Fig. 1: NanoLuc’s enzymatic activity and application scope.


  1. Hall, M. P. et al. Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem. Biol. 7, 1848–1857 (2012).

    Article  CAS  Google Scholar 

  2. Shimomura, O., Masugi, T., Johnson, F. H. & Haneda, Y. Properties and reaction mechanism of the bioluminescence system of the deep-sea shrimp Oplophorus gracilorostris. Biochemistry 17, 994–998 (1978).

    Article  CAS  Google Scholar 

  3. Inouye, S., Watanabe, K., Nakamura, H. & Shimomura, O. Secretional luciferase of the luminous shrimp Oplophorus graciliostris: cDNA cloning of a novel imidazopyrazinone luciferase. FEBS Lett. 481, 19–25 (2000).

    Article  CAS  Google Scholar 

  4. Inouye, S. et al. C6-Deoxy coelenterazine analogues as an efficient substrate for glow luminescence reaction of nanoKAZ: the mutated catalytic 19 kDa component of Oplophorus luciferase. Biochem. Biophys. Res. Commun. 437, 23–28 (2013).

    Article  CAS  Google Scholar 

  5. Suzuki, K. et al. Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat. Commun. 7, 13718 (2016).

    Article  CAS  Google Scholar 

  6. Dixon, A. S. et al. NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells. ACS Chem. Biol. 11, 400–408 (2015).

    Article  Google Scholar 

  7. Yang, J. et al. Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing. Nat. Commun. 7, 13268 (2016).

    Article  Google Scholar 

  8. Tomimuro, K. et al. Thread-based bioluminescent sensor for detecting multiple antibodies in a single drop of whole blood. ACS Sens. 5, 1786–1794 (2020).

    Article  CAS  Google Scholar 

  9. Berglund, K. et al. Luminopsins integrate opto- and chemogenetics by using physical and biological light sources for opsin activation. Proc. Natl Acad. Sci. USA. 113, 358–367 (2016).

    Article  Google Scholar 

  10. Mitouchkina, T. et al. Plants with genetically encoded autoluminescence. Nat. Biotechnol. 38, 944–946 (2020).

    Article  Google Scholar 

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Correspondence to Takeharu Nagai.

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Nagai, T., Hattori, M. Tiny but bright. Nat Rev Chem 6, 522–523 (2022).

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