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Nanosensors

New dimensions for fluorescence-based barcoding in complex mixtures

Combining single-molecule Förster resonance energy transfer (FRET) and fluorescence lifetime information inside an anti-Brownian electrokinetic (ABEL) trap makes it possible to distinguish dozens of biomolecules in a sample mixture. This method enables extensive barcoding of biomolecules with a minimal set of chemical components and opens up a path toward biomolecule quantification in complex mixtures.

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Fig. 1: Biomolecule detection through fluorescence-based barcoding in an anti-Brownian electrokinetic (ABEL) trap.

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Correspondence to Thorsten Hugel.

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Hugel, T. New dimensions for fluorescence-based barcoding in complex mixtures. Nat. Nanotechnol. (2024). https://doi.org/10.1038/s41565-024-01686-2

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