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New dimensions for fluorescence-based barcoding in complex mixtures

Combining single-molecule Förster resonance energy transfer (FRET) and fluorescence lifetime information inside an anti-Brownian electrokinetic (ABEL) trap makes it possible to distinguish dozens of biomolecules in a sample mixture. This method enables extensive barcoding of biomolecules with a minimal set of chemical components and opens up a path toward biomolecule quantification in complex mixtures.

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Fig. 1: Biomolecule detection through fluorescence-based barcoding in an anti-Brownian electrokinetic (ABEL) trap.


  1. Jain, A. et al. Nature 473, 484–488 (2011).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Chu, J. et al. Nat. Nanotechnol. (2024).

    Article  PubMed  Google Scholar 

  3. Agam, G. et al. Nat. Meth. 20, 523–535 (2023).

    Article  CAS  Google Scholar 

  4. Cohen, A. E. & Moerner, W. E. Appl. Phys. Lett. 86, 093109 (2005).

    Article  Google Scholar 

  5. Neuweiler, H., Doose, S. & Sauer, M. Proc. Natl Acad. Sci. 102, 16650–16655 (2005).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Myong, S. et al. Science 323, 1070–1074 (2009).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Correspondence to Thorsten Hugel.

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Hugel, T. New dimensions for fluorescence-based barcoding in complex mixtures. Nat. Nanotechnol. (2024).

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