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DNA nanostructures

Imaging DNA origami by fluorescence in situ hybridization

Conserved regions of the circular DNA sequence of the M13mp18 bacteriophage, which is used as a scaffold for DNA origami construction, are targeted with specific hybridization-chain-reaction probes. The probes enable sensitive detection of DNA origami nanostructures in cells, organoids and tissues to assess their biodistribution and stability.

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Fig. 1: A schematic overview of origamiFISH for in situ detection of DNA origami nanostructure and analysis by fluorescence imaging.

Adapted from ref. 6, Springer Nature Ltd


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Correspondence to Tania Patino.

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Patino, T. Imaging DNA origami by fluorescence in situ hybridization. Nat. Nanotechnol. 19, 1–2 (2024).

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