Abstract
Optical tweezers have emerged as a powerful tool for the non-invasive trapping and manipulation of colloidal particles and biological cells1,2. However, the diffraction limit precludes the low-power trapping of nanometre-scale objects. Substantially increasing the laser power can provide enough trapping potential depth to trap nanoscale objects. Unfortunately, the substantial optical intensity required causes photo-toxicity and thermal stress in the trapped biological specimens3. Low-power near-field nano-optical tweezers comprising plasmonic nanoantennas and photonic crystal cavities have been explored for stable nanoscale object trapping4,5,6,7,8,9,10,11,12,13. However, the demonstrated approaches still require that the object is trapped at the high-light-intensity region. We report a new kind of optically controlled nanotweezers, called opto-thermo-electrohydrodynamic tweezers, that enable the trapping and dynamic manipulation of nanometre-scale objects at locations that are several micrometres away from the high-intensity laser focus. At the trapping locations, the nanoscale objects experience both negligible photothermal heating and light intensity. Opto-thermo-electrohydrodynamic tweezers employ a finite array of plasmonic nanoholes illuminated with light and an applied a.c. electric field to create the spatially varying electrohydrodynamic potential that can rapidly trap sub-10 nm biomolecules at femtomolar concentrations on demand. This non-invasive optical nanotweezing approach is expected to open new opportunities in nanoscience and life science by offering an unprecedented level of control of nano-sized objects, including photo-sensitive biological molecules.
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Data availability
The datasets generated and/or analysed during the current study are available in the Harvard Dataverse repository (https://doi.org/10.7910/DVN/LFVQOD). Source data are provided with this paper.
Change history
29 September 2020
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
References
Neuman, K. C. & Block, S. M. Optical trapping. Rev. Sci. Instrum. 75, 2787–2809 (2004).
Ashkin, A. & Dziedzic, J. M. Optical trapping and manipulation of viruses and bacteria. Science 235, 1517–1520 (1987).
Blázquez-Castro, A. Optical tweezers: phototoxicity and thermal stress in cells and biomolecules. Micromachines 10, 507–549 (2019).
Juan, M. L., Gordon, R., Pang, Y., Eftekhari, F. & Quidant, R. Self-induced back-action optical trapping of dielectric nanoparticles. Nat. Phys. 5, 915–919 (2009).
Pang, Y. & Gordon, R. Optical trapping of 12 nm dielectric spheres using double-nanoholes in a gold film. Nano Lett. 11, 3763–3767 (2011).
Yoo, D. et al. Low-power optical trapping of nanoparticles and proteins with resonant coaxial nanoaperture using 10 nm gap. Nano Lett. 18, 3637–3642 (2018).
Saleh, A. A. E. & Dionne, J. A. Toward efficient optical trapping of sub-10-nm particles with coaxial plasmonic apertures. Nano Lett. 12, 5581–5586 (2012).
Zheng, Y. et al. Nano-optical conveyor belt, part II: demonstration of handoff between near-field optical traps. Nano Lett. 14, 2971–2976 (2014).
Roxworthy, B. J. et al. Application of plasmonic bowtie nanoantenna arrays for optical trapping, stacking, and sorting. Nano Lett. 12, 796–801 (2012).
Lin, L. et al. Opto-thermoelectric nanotweezers. Nat. Photon. 12, 195–201 (2018).
Mandai, S., Serey, X. & Erickson, D. Nanomanipulation using silicon photonic crystal resonators. Nano Lett. 10, 99–104 (2010).
Wang, K., Schonbrun, E., Steinvurzel, P. & Crozier, K. B. Trapping and rotating nanoparticles using a plasmonic nano-tweezer with an integrated heat sink. Nat. Commun. 2, 469 (2011).
Shoji, T. & Tsuboi, Y. Plasmonic optical tweezers toward molecular manipulation: tailoring plasmonic nanostructure, light source, and resonant trapping. J. Phys. Chem. Lett. 5, 2957–2967 (2014).
Ndukaife, J. C. et al. Long-range and rapid transport of individual nano-objects by a hybrid electrothermoplasmonic nanotweezer. Nat. Nanotechnol. 11, 53–59 (2016).
Ndukaife, J. C. et al. High-resolution large-ensemble nanoparticle trapping with multifunctional thermoplasmonic nanohole metasurface. ACS Nano 12, 5376–5384 (2018).
Ndukaife, J. C., Shalaev, V. M. & Boltasseva, A. Plasmonics—turning loss into gain. Science 351, 334–335 (2016).
Garcia-Guirado, J. et al. Overcoming diffusion-limited biosensing by electrothermoplasmonics. ACS Photonics 5, 3673–3679 (2018).
Hong, C., Yang, S. & Ndukaife, J. C. Optofluidic control using plasmonic TiN bowtie nanoantenna. Opt. Mater. Express 9, 953–964 (2019).
Dienerowitz, M., Mazilu, M., Reece, P. J., Krauss, T. F. & Dholakia, K. Optical vortex trap for resonant confinement of metal nanoparticles. Opt. Express 16, 4991–4999 (2008).
Fränzl, M. et al. Thermophoretic trap for single amyloid fibril and protein aggregation studies. Nat. Methods 16, 611–614 (2019).
Squires, T. M. & Bazant, M. Z. Induced-charge electro-osmosis. J. Fluid Mech. 509, 217–252 (2004).
Melcher, J. R. & Firebaugh, M. S. Traveling-wave bulk electroconvection induced across a temperature gradient. Phys. Fluids 10, 1178–1185 (1967).
Hatlo, M. M. & Lue, L. The role of image charges in the interactions between colloidal particles. Soft Matter 4, 1582–1596 (2008).
Nagpal, P., Lindquist, N. C., Oh, S. H. & Norris, D. J. Ultrasmooth patterned metals for plasmonics and metamaterials. Science 325, 594–597 (2009).
Ghosh, S. & Ghosh, A. All optical dynamic nanomanipulation with active colloidal tweezers. Nat. Commun. 10, 4191 (2019).
Pang, Y. & Gordon, R. Optical trapping of a single protein. Nano Lett. 12, 402–406 (2012).
Krishnan, M., Mojarad, N., Kukura, P. & Sandoghdar, V. Geometry-induced electrostatic trapping of nanometric objects in a fluid. Nature 467, 692–695 (2010).
Acknowledgements
The authors acknowledge financial support from the National Science Foundation (NSF ECCS-1933109) and Vanderbilt University. We thank A. Locke for providing the protein samples and K. Wang and C. Batista for help with the zeta potential measurements.
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J.C.N. conceived and guided the project. C.H. fabricated the samples and performed the experiments and the numerical simulations. S.Y. contributed to the wave-optics simulations. J.C.N and C.H. discussed the results and wrote the manuscript.
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Peer review information Nature Nanotechnology thanks Reuven Gordon and the other, anonymous, reviewer(s) for their contribution to the peer review of this work
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Supplementary information
Supplementary Information
Supplementary Figs. 1–2, discussion and refs. 1–5.
Supplementary Video 1
Fast transport, trapping and release of a single BSA protein molecule.
Supplementary Video 2
Dynamic manipulation of a single BSA protein molecule.
Supplementary Video 3
Frequency-dependent tuning of trapping position.
Supplementary Video 4
Sorting 20 nm polystyrene particles from a mixture of 100 nm and 20 nm polystyrene particles by changing the a.c. frequency.
Source data
Source Data Fig. 2
Excel data of particle positions for different a.c. frequencies.
Source Data Fig. 4
Excel data of particle displacements from the edge of the nanohole array for different a.c. frequencies; simulation data for the fluid’s radial velocity as a function of position for different a.c. frequencies; and Excel data of particle positions for different a.c. frequencies.
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Hong, C., Yang, S. & Ndukaife, J.C. Stand-off trapping and manipulation of sub-10 nm objects and biomolecules using opto-thermo-electrohydrodynamic tweezers. Nat. Nanotechnol. 15, 908–913 (2020). https://doi.org/10.1038/s41565-020-0760-z
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DOI: https://doi.org/10.1038/s41565-020-0760-z
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