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A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing


Delivery technologies for the CRISPR-Cas9 (CRISPR, clustered regularly interspaced short palindromic repeats) gene editing system often require viral vectors, which pose safety concerns for therapeutic genome editing1. Alternatively, cationic liposomal components or polymers can be used to encapsulate multiple CRISPR components into large particles (typically >100 nm diameter); however, such systems are limited by variability in the loading of the cargo. Here, we report the design of customizable synthetic nanoparticles for the delivery of Cas9 nuclease and a single-guide RNA (sgRNA) that enables the controlled stoichiometry of CRISPR components and limits the possible safety concerns in vivo. We describe the synthesis of a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating, called a nanocapsule (NC), around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. The NC is synthesized by in situ polymerization, has a hydrodynamic diameter of 25 nm and can be customized via facile surface modification. NCs efficiently generate targeted gene edits in vitro without any apparent cytotoxicity. Furthermore, NCs produce robust gene editing in vivo in murine retinal pigment epithelium (RPE) tissue and skeletal muscle after local administration. This customizable NC nanoplatform efficiently delivers CRISPR RNP complexes for in vitro and in vivo somatic gene editing.

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Fig. 1: Design, synthesis and optimization of NCs.
Fig. 2: Stability, uptake and toxicity characteristics of NCs within human cells in vitro.
Fig. 3: Decoration of NCs with cell penetrating peptides increases on-target genome editing efficiency in vitro within human cell lines.
Fig. 4: NCs can induce efficient genome editing in vivo within Ai14 reporter mice.

Data availability

The data that support the plots within this paper and other findings of this study are available from the corresponding authors upon reasonable request.


  1. Yin, H., Kauffman, K. J. & Anderson, D. G. Delivery technologies for genome editing. Nat. Rev. Drug Discov. 16, 387–399 (2017).

    Article  CAS  Google Scholar 

  2. Liu, J. et al. Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells. Nat. Protocols 10, 1842–1859 (2015).

    Article  CAS  Google Scholar 

  3. Li, L., He, Z., Wei, X., Gao, G. & Wei, Y. Challenges in CRISPR/CAS9 delivery: potential roles of nonviral vectors. Hum. Gene Ther. 26, 452–462 (2015).

    Article  CAS  Google Scholar 

  4. Mout, R., Ray, M., Lee, Y. W., Scaletti, F. & Rotello, V. M. In vivo delivery of CRISPR/Cas9 for therapeutic gene editing: progress and challenges. Bioconjugate Chem. 28, 880–884 (2017).

    Article  CAS  Google Scholar 

  5. Wang, H. X. et al. CRISPR/Cas9-based genome editing for disease modeling and therapy: challenges and opportunities for nonviral delivery. Chem. Rev. 117, 9874–9906 (2017).

    Article  CAS  Google Scholar 

  6. Li, L., Hu, S. & Chen, X. Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities. Biomaterials 171, 207–218 (2018).

    Article  CAS  Google Scholar 

  7. Rui, Y., Wilson, D. R. & Green, J. J. Non-viral delivery to enable genome editing. Trends Biotechnol. 37, 281–293 (2019).

    Article  CAS  Google Scholar 

  8. Gu, Z. et al. Protein nanocapsule weaved with enzymatically degradable polymeric network. Nano Lett. 9, 4533–4538 (2009).

    Article  CAS  Google Scholar 

  9. Zhao, M. et al. Clickable protein nanocapsules for targeted delivery of recombinant p53 protein. J. Am. Chem. Soc. 136, 15319–15325 (2014).

    Article  CAS  Google Scholar 

  10. Yan, M. et al. A novel intracellular protein delivery platform based on single-protein nanocapsules. Nat. Nanotechnol. 5, 48–53 (2010).

    Article  CAS  Google Scholar 

  11. Putnam, D., Gentry, C. A., Pack, D. W. & Langer, R. Polymer-based gene delivery with low cytotoxicity by a unique balance of side-chain termini. Proc. Natl Acad. Sci. USA 98, 1200–1205 (2001).

    Article  CAS  Google Scholar 

  12. Meng, F., Cheng, R., Deng, C. & Zhong, Z. Intracellular drug release nanosystems. Mater. Today 15, 436–442 (2012).

    Article  CAS  Google Scholar 

  13. Liu, F. et al. PKM2 methylation by CARM1 activates aerobic glycolysis to promote tumorigenesis. Nat. Cell Biol. 19, 1358–1370 (2017).

    Article  CAS  Google Scholar 

  14. Mout, R. et al. Direct cytosolic delivery of CRISPR/Cas9-ribonucleoprotein for efficient gene editing. ACS Nano 11, 2452–2458 (2017).

    Article  CAS  Google Scholar 

  15. Carlson-Stevermer, J. et al. High-content analysis of CRISPR-Cas9 gene-edited human embryonic stem cells. Stem Cell Rep. 6, 109–120 (2016).

    Article  CAS  Google Scholar 

  16. Carlson-Stevermer, J. et al. Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing. Nat. Commun. 8, 1711 (2017).

    Article  Google Scholar 

  17. Elzes, M. R., Akeroyd, N., Engbersen, J. F. & Paulusse, J. M. Disulfide-functional poly(amido amine)s with tunable degradability for gene delivery. J. Control. Release 244, 357–365 (2016).

    Article  CAS  Google Scholar 

  18. Staahl, B. T. et al. Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes. Nat. Biotechnol. 35, 431–434 (2017).

    Article  CAS  Google Scholar 

  19. Payton, N. M., Wempe, M. F., Xu, Y. & Anchordoquy, T. J. Long-term storage of lyophilized liposomal formulations. J. Pharm. Sci. 103, 3869–3878 (2014).

    Article  CAS  Google Scholar 

  20. Chen, G. et al. Multi-functional self-fluorescent unimolecular micelles for tumor-targeted drug delivery and bioimaging. Biomaterials 47, 41–50 (2015).

    Article  CAS  Google Scholar 

  21. Sun, J. et al. CRISPR/Cas9 editing of APP C-terminus attenuates β-cleavage and promotes α-cleavage. Nat. Commun. 10, 53 (2019).

    Article  Google Scholar 

  22. Berger, W., Kloeckener-Gruissem, B. & Neidhardt, J. The molecular basis of human retinal and vitreoretinal diseases. Prog. Retin. Eye Res. 29, 335–375 (2010).

    Article  CAS  Google Scholar 

  23. Maeder, M. L. et al. Development of a gene-editing approach to restore vision loss in Leber congenital amaurosis type 10. Nat. Med. 25, 229–233 (2019).

    Article  CAS  Google Scholar 

  24. Sun, D. et al. Targeted multifunctional lipid ECO plasmid DNA nanoparticles as efficient non-viral gene therapy for Leber’s congenital amaurosis. Mol. Ther. Nucleic Acids 7, 42–52 (2017).

    Article  CAS  Google Scholar 

  25. Marmor, M. F. Control of subretinal fluid: experimental and clinical studies. Eye 4, 340–344 (1990).

    Article  Google Scholar 

  26. Strauss, O. The retinal pigment epithelium in visual function. Physiol. Rev. 85, 845–881 (2005).

    Article  CAS  Google Scholar 

  27. Mao, Y. & Finnemann, S. C. in Retinal Degeneration (eds Weber, B. & Langmann, T.) 285–295 (Springer, 2012).

  28. Mazzoni, F., Mao, Y. & Finnemann, S. C. in Retinal Degeneration (eds Weber, B. & Langmann, T.) 95–108 (Springer, 2019).

  29. Steyer, B. et al. High content analysis platform for optimization of lipid mediated CRISPR-Cas9 delivery strategies in human cells. Acta Biomater. 34, 143–158 (2016).

    Article  CAS  Google Scholar 

  30. Zuris, J. A. et al. Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. Nat. Biotechnol. 33, 73–80 (2015).

    Article  CAS  Google Scholar 

  31. Harkness, T. et al. High‐content imaging with micropatterned multiwell plates reveals influence of cell geometry and cytoskeleton on chromatin dynamics. Biotechnol. J. 10, 1555–1567 (2015).

    Article  CAS  Google Scholar 

  32. Park, J., Lim, K., Kim, J. S. & Bae, S. Cas-analyzer: an online tool for assessing genome editing results using NGS data. Bioinformatics 33, 286–288 (2017).

    Article  CAS  Google Scholar 

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We thank the James Thomson lab for the use of their BD FACSCanto II, and the University of Wisconsin Biotechnology Center for providing facilities and services. We thank Aldevron for supplying reagents and technical support. We are grateful to Q. Chang who provided us with the Ai14 tdTomato transgenic mice used for the initial evaluation. We acknowledge the generous financial support from the National Institute for Health (1-UG3-NS-111688-01, R01EY024995, 1R35GM119644, R01NS091540, R01HL143469 and R01 HL129785), the National Science Foundation (CBET-1350178 and CBET-1645123), the Wisconsin Alumni Research Foundation and the Wisconsin Institute for Discovery. The authors also acknowledge financial support from the University of Wisconsin–Madison.

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Authors and Affiliations



G.C., A.A.A., Y.W., K.S. and S.G. conceived and designed the project, G.C., A.A.A., Y.W., R.X., M.S., S.R. and P.K.S. performed the experiments, all the authors analysed the data and G.C., A.A.A., Y.W., P.K.S., B.R.P., M.S., K.S. and S.G. co-wrote the paper.

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Correspondence to Krishanu Saha or Shaoqin Gong.

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G.C., A.A.A., Y.W., R.X., K.S. and S.G. have filed a patent application on this work.

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Chen, G., Abdeen, A.A., Wang, Y. et al. A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing. Nat. Nanotechnol. 14, 974–980 (2019).

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