TZM-bl cells transiently expressing Sec24C-mCherry were infected with INmNG-labelled HIV-1 bearing WT CA or the N74D CA mutant. At 2 hpi, cells were analysed for colocalization of INmNG-labelled WT or mutant N74D virus with Sec24C. (a) Representative images and (b) quantification of Sec24C-mCherry signal associated with INmNG-labelled puncta. The results are from n = 1202 WT/CA puncta from 43 cells and n = 1202 N74D/CA puncta from 25 cells. Colocalization (%) of the Sec24C-mCherry signal with INmNG puncta is shown in the graph. The averaged data are representative of two independent experiments. Statistical significance was determined by two-tailed student t-test. Arrows in (a) point to Sec24C-mCherry puncta colocalized with INmNG spots. Scale bar is 5 µm.