Extended Data Fig. 2: Effects of Trim-Sec24C196–314 on different retroviruses. | Nature Microbiology

Extended Data Fig. 2: Effects of Trim-Sec24C196–314 on different retroviruses.

From: Sec24C is an HIV-1 host dependency factor crucial for virus replication

Extended Data Fig. 2

(a) Representative immunoblot showing the stable expression of Trim-HA (blue line), Trim-CPSF6261–358 (orange line) and Trim- Sec24C196–314 (grey line) in HeLa cells. (b-h) Infections of HeLa cells stably expressing indicated Trim-fusion constructs with following VSV-G pseudotyped viruses: HIV-1NL4-3 (b), SIVmac239 (c), SIVstm (d), SIVsmE041 (e), FIV (f), EIAV (g), MLV (h). Infection levels of HIV-1-scarlet, SIVstm-eGFP, SIVmac239- eGFP, SIVsmE041-eGFP, FIV-eGFP, EIAV-eGFP, and MLV-RFP were determined by FACS. The averaged data (mean values ± SD) from three independent experiments are shown with exception of FIV infection in Trim-HA overexpressing and EIAV infection in Trim-CPSF6261–358 overexpressing Hela cells which were obtained from two independent experiments.

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