Extended Data Fig. 1: Identification of Sec24C as HIV-1 CA-interacting partner. | Nature Microbiology

Extended Data Fig. 1: Identification of Sec24C as HIV-1 CA-interacting partner.

From: Sec24C is an HIV-1 host dependency factor crucial for virus replication

Extended Data Fig. 1

a, Experimental design of the MS-based proteomics approach (See Online Methods for details). b, Representative immunoblots showing CA tubes mediated pull-downs of endogenous Sec24C from THP1 cells without (lanes 1–5) or with (lanes 6–10) IFN treatments. Lanes 1 and 6: lysates, 2 and 7: supernatant or unbound fraction after pulldown in the absence of CA tubes, 3 and 8: supernatant or unbound fraction after pulldown in the presence of CA tubes; 4 and 9: pelleted or bound fraction in the absence of CA tubes; 5 and 10: pelleted or bound fraction in the presence of CA tubes.

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