Single-cycle replication assays were performed in WT, Sec24C KO, KO + Sec24C, or KO + Sec24C(ΔFG) Jurkat cells. Infection levels (normalized to WT Jurkat cells) of VSV-G pseudotyped eGFP reporter viruses: HIV-1 (a), SIVmac239 (b), SIVstm (c), SIVsmE041 (d), FIV (e), EIAV (f), MLV (g) were measured by FACS. The data (mean values ± SD) from three independent experiments are shown. (h) P-values. Statistically significant changes were observed for all lentiviruses, when their infection levels were compared for KO vs WT, KO + WT vs KO and KO + ΔFG vs KO + WT. In contrast, no statistically significant differences were seen for FIV or MLV across these cell lines. Sec24C KO resulted in slight but statistically significant increase of EIAV infection (KO vs WT). This effect is opposite to Sec24C KO effects on primate lentiviruses. Furthermore, in complete contrast with primate lentiviruses, no detectable differences were observed for EIAV in KO + ΔFG vs KO + WT cells. Statistical significance was determined by two-tailed student t-test.