(a-c) Stability of CA tubes as a function of time. CA tubes were preformed at 2 M NaCl, pelleted and then resuspended in a 1 M NaCl containing buffer to induce disassembly of the tubes (a). GST-Sec24C196–314(ΔFG) (b) or GST-Sec24C196–314 (c) was added to pre-formed CA tubes and then reactions were diluted to 1 M NaCl buffer. The mixtures were centrifuged at indicated time points and pellets were analysed by SDS–PAGE. (d) Quantification of the results in (a–c). The data (mean values ± SD) from three independent experiments are shown.