Extended Data Fig. 8: Effects of GST-Sec24C196–314 on the stability of CA tubes. | Nature Microbiology

Extended Data Fig. 8: Effects of GST-Sec24C196–314 on the stability of CA tubes.

From: Sec24C is an HIV-1 host dependency factor crucial for virus replication

Extended Data Fig. 8

(a-c) Stability of CA tubes as a function of time. CA tubes were preformed at 2 M NaCl, pelleted and then resuspended in a 1 M NaCl containing buffer to induce disassembly of the tubes (a). GST-Sec24C196–314(ΔFG) (b) or GST-Sec24C196–314 (c) was added to pre-formed CA tubes and then reactions were diluted to 1 M NaCl buffer. The mixtures were centrifuged at indicated time points and pellets were analysed by SDS–PAGE. (d) Quantification of the results in (a–c). The data (mean values ± SD) from three independent experiments are shown.

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