Fig. 5: Comparison of chimeric and full-length clade 2 and 3 spikes. | Nature Microbiology

Fig. 5: Comparison of chimeric and full-length clade 2 and 3 spikes.

From: Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses

Fig. 5

a, Design of full-length spike sequences from As6526 (clade 2) and BM48-31 (clade 3). b, Western blot of producer cell lysates and concentrated pseudotype particles. c, Pseudotypes were left untreated or treated with trypsin and subsequently used to infect Huh-7.5 cells. d, Pseudotypes with the indicated spike constructs were left untreated or treated with trypsin and subsequently used to infect BHK cells expressing human ACE2. The data are representative of three technical replicates. Vertical bars indicate s.d. and horizontal bars indicate mean values of all three replicates.

Source data

Back to article page