a, Growth curves of PM6 in M9-acetate (magenta), M9-glucose (orange), and EZRDM (olive). Data are presented as mean ± s.d. from three independent experiments. b, Representative kymographs of the septum closure progress in PM6 cells growing in the indicated media, as probed by mNeonGreen-ZapA fluorescence. Representative micrographs were from two independent experiments. Scale bars: 200 nm. c, Relationship between the cell growth rate (Vg, estimated from a) and the constriction rate (Vc, estimated from kymographs as in b). The boxes indicate the ± SEM, whiskers the 10–90 percentiles, the midline indicates median, the square indicates mean. b,c, For the sample size of condition see Supplementary Table 8.