Extended Data Fig. 4: IL-36γ promotes killing of K. pneumoniae by macrophages. | Nature Microbiology

Extended Data Fig. 4: IL-36γ promotes killing of K. pneumoniae by macrophages.

From: Commensal Bacteroidetes protect against Klebsiella pneumoniae colonization and transmission through IL-36 signalling

Extended Data Fig. 4

Bone marrow derived-macrophages (a,b) and J774 macrophages (c,d) were incubated with K. pneumoniae ST258 (a,c) and OXA-48 (b,d) (at an MOI of 1:10) for 3 hours. Indicated groups were treated with recombinant IL-36γ (50 ng/ml) for 3 hours prior to infection. Bacterial viability was then determined and expressed relative to the initial number of bacteria in the assay (n=5 independent determinations). Statistical comparisons were by Student’s t-test (two-tailed), error bars are standard deviation, bars represent mean values.

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