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Host NLRP6 exacerbates graft-versus-host disease independent of gut microbial composition


Host NOD-like receptor family pyrin domain-containing 6 (NLRP6) regulates innate immune responses and gastrointestinal homeostasis. Its protective role in intestinal colitis and tumorigenesis is dependent on the host microbiome. Host innate immunity and microbial diversity also play a role in the severity of allogeneic immune-mediated gastrointestinal graft-versus-host disease (GVHD), the principal toxicity after allogeneic haematopoietic cell transplantation. Here, we examined the role of host NLRP6 in multiple murine models of allogeneic bone marrow transplantation. In contrast to its role in intestinal colitis, host NLRP6 aggravated gastrointestinal GVHD. The impact of host NLRP6 deficiency in mitigating GVHD was observed regardless of co-housing, antibiotic treatment or colonizing littermate germ-free wild-type and NLRP6-deficient hosts with faecal microbial transplantation from specific pathogen-free wild-type and Nlrp6−/− animals. Chimaera studies were performed to assess the role of NLRP6 expression on host haematopoietic and non-haematopoietic cells. The allogeneic [B6Ly5.2 → Nlrp6−/−] animals demonstrated significantly improved survival compared to the allogeneic [B6Ly5.2 → B6] animals, but did not alter the therapeutic graft-versus-tumour effects after haematopoietic cell transplantation. Our results unveil an unexpected, pathogenic role for host NLRP6 in gastrointestinal GVHD that is independent of variations in the intestinal microbiome and in contrast to its well-appreciated microbiome-dependent protective role in intestinal colitis and tumorigenesis.

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Fig. 1: The absence of NLRP6 in hosts ameliorates GVHD in experimental BMT models.
Fig. 2: Nlrp6−/− animals showed similar expansion of donor-derived myeloid cells with B6 WT animals.
Fig. 3: Nlrp6−/− animals showed less GVHD severity after co-housing or antibiotic treatments.
Fig. 4: Absence of NLRP6 in host haematopoietic-derived APCs is dispensable for reducing GVHD severity and mortality.
Fig. 5: Absence of NLRP6 on host non-haematopoietic cells plays an important role in ameliorating GVHD severity and mortality.
Fig. 6: Absence of NLRP6 in IECs enhances gut homeostasis after allo-BMT.

Data availability

The raw sequencing reads have been deposited at the NCBI Short Read Archive under BioProject ID PRJNA491725. Metabolomic data, mass spectral analytical parameters and spectral raw data from the study and metadata have been deposited in the NIH Common Fund’s Data Repository and Coordinating Center under Metabolomics Workbench Project ID PR000728.


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This work was supported by the National Institutes of Health grants AI-075284 (P.R.) and HL-090775 (P.R.), the American Society of Blood and Marrow Transplantation New Investigator Award (T.T.), the JSPS Postdoctoral Fellowships for Research Abroad (H.F.) and the YASUDA Medical Foundation Grants for Research Abroad (H.F.). This research was supported by work performed by the University of Michigan Microbial Systems Molecular Biology Laboratory.

Author information




T.T. designed and performed the experiments, analysed the data and wrote the paper. H.F., C.R. and M.R. performed the experiments, analysed the data and wrote the paper. H.T. and C.Z. performed the experiments and analysed the data. C.L. performed the experiments and histopathological analysis. A.V.M., J.B., K.O.-W., I.M., S.B., Y.S., D.P., J.W., I.H., S.K., J.C., S.S. and S.P. performed the experiments. G.C. analysed the data, contributed reagents and wrote the paper. P.R. designed the experiments, analysed the data and wrote the paper.

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Correspondence to Grace Chen or Pavan Reddy.

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Supplementary Information

Supplementary Figures 1–5.

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A dataset showing the exact P values for all graphs in this manuscript.

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Toubai, T., Fujiwara, H., Rossi, C. et al. Host NLRP6 exacerbates graft-versus-host disease independent of gut microbial composition. Nat Microbiol 4, 800–812 (2019).

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