Abstract

Filoviruses, especially Ebola virus (EBOV) and Marburg virus (MARV), are notoriously pathogenic and capable of causing severe haemorrhagic fever diseases in humans with high lethality1,2. The risk of future outbreaks is exacerbated by the discovery of other bat-borne filoviruses of wide genetic diversity globally3,4,5. Here we report the characterization of a phylogenetically distinct bat filovirus, named Měnglà virus (MLAV). The coding-complete genome of MLAV shares 32–54% nucleotide sequence identity with known filoviruses. Phylogenetic analysis places this new virus between EBOV and MARV, suggesting the need for a new genus taxon. Importantly, despite the low amino acid sequence identity (22–39%) of the glycoprotein with other filoviruses, MLAV is capable of using the Niemann–Pick C1 (NPC1) as entry receptor. MLAV is also replication-competent with chimeric MLAV mini-genomes containing EBOV or MARV leader and trailer sequences, indicating that these viruses are evolutionally and functionally closely related. Finally, MLAV glycoprotein-typed pseudo-types transduced cell lines derived from humans, monkeys, dogs, hamsters and bats, implying a broad species cell tropism with a high risk of interspecies spillover transmission.

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Data availability

The data that support the findings of this study are available from the corresponding author upon request. The GenBank accession number for the genome sequence of MLAV is KX371887.

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Acknowledgements

This study was supported in part by the strategic priority research programme of the Chinese Academy of Sciences (no. XDB29010000 to Z-L.S.), Singapore National Research Foundation grants (nos. NRF2012NRF-CRP001-056 and NRF2016NRF-NSFC002-013 to L.-F.W.) and a Singapore MINDEF grant (no. MINDEF-NUS-DIRP/2015/04 to L.-F.W. and D.E.A.). X.L.Y. is supported by the Visiting Scientist Fellowship from the Chinese Academy of Sciences. We thank T. Hoenen and H. Feldmann for providing plasmids from which the relevant EBOV genes were derived for this study.

Author information

Author notes

  1. These authors contributed equally: Xing-Lou Yang, Chee Wah Tan.

Affiliations

  1. CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China

    • Xing-Lou Yang
    • , Ren-Di Jiang
    • , Bei Li
    • , Wei Zhang
    • , Yan Zhu
    • , Peng Zhou
    • , Xiang-Ling Liu
    • , Wuxiang Guan
    •  & Zheng-Li Shi
  2. Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore

    • Xing-Lou Yang
    • , Chee Wah Tan
    • , Danielle E. Anderson
    • , Xiao Fang Lim
    •  & Lin-Fa Wang
  3. University of Chinese Academy of Sciences, Beijing, China

    • Ren-Di Jiang
  4. Guangdong Institute of Applied Biological Resources, Guangzhou, China

    • Libiao Zhang
  5. Wuhan University, Wuhan, China

    • Shi-Yue Li
  6. Yunnan Institute of Endemic Diseases Control and Prevention, Dali, China

    • Yun-Zhi Zhang
  7. Dali University, Dali, China

    • Yun-Zhi Zhang

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Contributions

X.-L.Y., L.-F.W. and Z.-L.S. conceived the study. X.-L.Y., C.W.T., R.-D.J., B.L., W.Z., Y.Z., X.F.L., P.Z., X.-L.L., L.Z., S.-Y.L. and Y.-Z.Z. conducted the experiments. W.G., Z.-L.S. and L.-F.W. provided resources and reagents. X.-L.Y., C.W.T. and D.E.A. analysed the data. X.-L.Y., C.W.T., D.E.A., Z.-L.S. and L.-F.W. prepared the manuscript. All authors read, edited and approved the manuscript.

Competing interests

The authors declare no competing interests.

Corresponding authors

Correspondence to Lin-Fa Wang or Zheng-Li Shi.

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DOI

https://doi.org/10.1038/s41564-018-0328-y