Here we describe the complete genome of a new ebolavirus, Bombali virus (BOMV) detected in free-tailed bats in Sierra Leone (little free-tailed (Chaerephon pumilus) and Angolan free-tailed (Mops condylurus)). The bats were found roosting inside houses, indicating the potential for human transmission. We show that the viral glycoprotein can mediate entry into human cells. However, further studies are required to investigate whether exposure has actually occurred or if BOMV is pathogenic in humans.
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Burk, R. et al. Neglected filoviruses. FEMS Microbiol. Rev. 40, 494–519 (2016).
Marí Saéz, A. et al. Investigating the zoonotic origin of the West African Ebola epidemic.EMBO Mol. Med. 7, 17–23 (2015).
Lo, T. Q., Marston, B. J., Dahl, B. A. & De Cock, K. M. Ebola: anatomy of an epidemic. Annu. Rev. Med. 68, 359–370 (2017).
Leroy, E. M. et al. Fruit bats as reservoirs of Ebola virus. Nature 438, 575–576 (2005).
Pourrut, X. et al. Spatial and temporal patterns of Zaire ebolavirus antibody prevalence in the possible reservoir bat species. J. Infect. Dis. 196, S176–S183 (2007).
Hayman, D. T. et al. Ebola virus antibodies in fruit bats, Ghana, West Africa. Emerg. Infect. Dis. 18, 1207–1209 (2012).
Yuan, J. et al. Serological evidence of ebolavirus infection in bats, China. Virol. J. 9, 236 (2012).
Negredo., A. et al. Discovery of an ebolavirus-like filovirus in Europe. PLoS Pathog. 7, e1002304 (2011).
Jayme, S. I. et al. Molecular evidence of Ebola Reston virus infection in Philippine bats. Virol. J. 12, 107 (2015).
Bào, Y. et al. Implementation of objective PASC-derived taxon demarcation criteria for official classification of filoviruses. Viruses 9, 106 (2017).
Swanepoel, R. et al. Experimental inoculation of plants and animals with Ebola virus. Emerg. Infect. Dis. 2, 321–325 (1996).
Leendertz, S. A. J. Testing new hypotheses regarding ebolavirus reservoirs. Viruses 8, 30 (2016).
Carette, J. E. et al. Ebola virus entry requires the cholesterol transporter Niemann-Pick C1. Nature 477, 340–343 (2011).
Côté, M. et al. Small molecule inhibitors reveal Niemann-Pick C1 is essential for Ebola virus infection. Nature 477, 344–348 (2011).
Miller, E. H. et al. Ebola virus entry requires the host-programmed recognition of an intracellular receptor. EMBO J. 31, 1947–1960 (2012).
Ng, M. et al. Filovirus receptor NPC1 contributes to species-specific patterns of ebolavirus susceptibility in bats. eLife 4, e11785 (2015).
Bornholdt, Z. A. et al. Host-primed Ebola virus GP exposes a hydrophobic NPC1 receptor-binding pocket, revealing a target for broadly neutralizing antibodies. mBio 7, e02154-15 (2016).
Wang, H. et al. Ebola viral glycoprotein bound to its endosomal receptor Niemann-Pick C1.Cell 164, 258–268 (2016).
Pappalardo, M. et al. Conserved differences in protein sequence determine the human pathogenicity of Ebolaviruses. Sci. Rep. 6, 23743 (2016).
Miranda, M. E. & Miranda, N. L. Reston ebolavirus in humans and animals in the Philippines: a review. J. Infect. Dis. 204, S757–S760 (2011).
Bale, S. et al. Ebolavirus VP35 coats the backbone of double-stranded RNA for interferon antagonism. J. Virol. 87, 10385–10388 (2013).
Reid, S. P. et al. Ebola virus VP24 binds karyopherin alpha1 and blocks STAT1 nuclear accumulation. J. Virol. 80, 5156–5167 (2006).
Volchkov, V. E., Blinov, V. M. & Netesov, S. V. The envelope glycoprotein of Ebola virus contains an immunosuppressive-like domain similar to oncogenic retroviruses. FEBS Lett. 305, 181–184 (1992).
Schoepp, R. J., Rossi, C. A., Khan, S. H., Goba, A. & Fair, J. N. Undiagnosed acute viral febrile illnesses, Sierra Leone. Emerg. Infect. Dis. 20, 1176–1182 (2014).
Towner, J. S. et al. Marburg virus infection detected in a common African bat. PLoS ONE 2, e764 (2007).
Yang, X. L. et al. Genetically diverse filoviruses in Rousettus and Eonycteris spp. bats, China, 2009 and 2015. Emerg. Infect. Dis. 23, 482–486 (2017).
Amman, B. R. et al. Marburgvirus resurgence in Kitaka Mine bat population after extermination attempts, Uganda.Emerg. Infect. Dis. 20, 1761–1764 (2014).
Townzen, J. S., Brower, A. V., & Judd, D. D. Identification of mosquito bloodmeals using mitochondrial cytochrome oxidase subunit I and cytochrome b gene sequences.Med. Vet. Entomol. 22, 386–393 (2008).
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol. 3, 294–299 (1994).
Towner, J. S. et al. Rapid diagnosis of Ebola hemorrhagic fever by reverse transcription-PCR in an outbreak setting and assessment of patient viral load as a predictor of outcome.J. Virol. 78, 4330–4341 (2004).
Jääskeläinen, A. J. et al. Development and evaluation of a real-time EBOV-L-RT-qPCR for detection of Zaire ebolavirus.J. Clin. Virol. 67, 56–58 (2015).
Anthony, S. J. et al. Further evidence for bats as the evolutionary source of Middle East respiratory syndrome coronavirus. mBio 8, e00373-17 (2017).
Briese, T. et al. Virome capture sequencing enables sensitive viral diagnosis and comprehensive virome analysis. mBio 6, e01491-15 (2015).
Spence, J. S., Krause, T. B., Mittler, E., Jangra, R. K. & Chandran, K. Direct visualization of Ebola virus fusion triggering in the endocytic pathway. mBio 7, e01857-15 (2016).
Wong, A. C., Sandesara, R. G., Mulherkar, N., Whelan, S. P. & Chandran, K. A forward genetic strategy reveals destabilizing mutations in the Ebolavirus glycoprotein that alter its protease dependence during cell entry. J. Virol. 84, 163–175 (2010).
Whelan, S. P. J., Ball, L. A., Barr, J. N. & Wertz, G. T. Efficient recovery of infectious vesicular stomatitis virus entirely from cDNA clones. Proc. Natl Acad. Sci. USA 92, 8388–8392 (1995).
Kleinfelter, L. M. et al. Haploid genetic screen reveals a profound and direct dependence on cholesterol for hantavirus membrane fusion. mBio 6, e00801 (2015).
Chandran, K., Sullivan, N. J., Felbor, U., Whelan, S. P. & Cunningham, J. M. Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection. Science 308, 1643–1645 (2005).
Ng, M. et al. Cell entry by a novel European filovirus requires host endosomal cysteine proteases and Niemann-Pick C1.Virology 468–470, 637–646 (2014).
Petrey, D. et al. Using multiple structure alignments, fast model building, and energetic analysis in fold recognition and homology modeling.Proteins 53, S430–S435 (2003).
Li, W., Jaroszewski, L. & Godzik, A. Clustering of highly homologous sequences to reduce the size of large protein databases. Bioinformatics 17, 282–283 (2001).
King, D. P. et al. Humoral immune responses to phocine herpesvirus-1 in Pacific harbor seals (Phoca vitulina richardsii) during an outbreak of clinical disease.Vet. Microbiol. 80, 1–8 (2001).
We thank the government of Sierra Leone for permission to conduct this work; the Sierra Leone district and community stakeholders for their support and for allowing us to perform sampling in their districts and communities; the Bombali Ministry of Health and Sanitation and Ministry of Agriculture district officers, field teams and regional lead including M. LeBreton, F. Jean Louis, K. Kargbo, L.A.M. Kenny, V. Lungay, W. Robert, E. Amara, D. Kargbo, V. Merewhether-Thompson, M. Kanu, E. Lavallie, A. Bangura, M. Turay, F.V. Bairoh, M. Sinnah and S. Yonda for performing sample collection; Yongai Saah Bona for administrative and logistic support; laboratory staff for assistance with processing the samples, including M. Coomber and O. Kanu (University of Makeni) and V. Ontiveros (UC Davis); T. O’Rourke, D. O’ Rourke (Metabiota) and D. Greig (UC Davis) for assistance with data entry, B. Lee for bioinformatics assistance and J. Morrison and A. Rasmussen for technical guidance (Columbia University); N. Randhawa for map graphics (UC Davis); and W. Karesh and J. Epstein (EcoHealth Alliance) for global input into study design. This study was made possible by the generous support of the American people through the United States Agency for International Development (USAID) Emerging Pandemic Threats PREDICT project (cooperative agreement number GHN-A-OO-09-00010-00) and by support from the National Institutes of Health (GM030518, S10OD012351, S10OD021764 and GM109018-05).
The authors declare no competing interests.
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Goldstein, T., Anthony, S.J., Gbakima, A. et al. The discovery of Bombali virus adds further support for bats as hosts of ebolaviruses. Nat Microbiol 3, 1084–1089 (2018). https://doi.org/10.1038/s41564-018-0227-2
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