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Recognizing the reagent microbiome

A noticeable part of the microbiome literature, especially that working with low-biomass samples, is plagued by reagent contamination. Here we describe visual, statistical, methodical and ecological techniques to facilitate recognition of signals that represent contamination.

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Acknowledgements

The work was supported by the Medical Research Council (UK; G1100221) and the National Institute for Health Research (NIHR) Cambridge Comprehensive Biomedical Research Centre (Women’s Health theme).

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Competing interests

The authors declare no competing interests.

Correspondence to Julian Parkhill.

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Further reading

Fig. 1: Reagent contamination recognition strategies.