Discovering antibiotics from the global microbiome

Metagenomic sequencing, bioinformatic analysis and heterologous expression of an orphan biosynthetic gene cluster widely found in the environment led to the discovery and structural characterization of a novel group of calcium-dependent antibiotics hidden in plain sight.

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Fig. 1: Sequence-guided characterization of the malacidins.


  1. 1.

    Allen, H. K. et al. Nat. Rev. Microbiol. 8, 251–259 (2010).

  2. 2.

    Hover, B. M. et al. Nat. Microbiol. (2018).

  3. 3.

    Robbel, L. & Marahiel, M. A. J. Biol. Chem. 285, 27501–27508 (2010).

  4. 4.

    Schneider, T. et al. Antimicrob. Agents Chemother. 53, 1610–1618 (2009).

  5. 5.

    Ling, L. L. et al. Nature 517, 455–459 (2015).

  6. 6.

    Breukink, E. & de Kruijff, B. Nat. Rev. Drug Discov. 5, 321–332 (2006).

  7. 7.

    Walsh, C. T., Fisher, S. L., Park, I.-S., Prahalad, M. & Wu, Z. Chem. Biol. 3, 21–28 (1996).

  8. 8.

    Blin, K., Kim, H. U., Medema, M. H. & Weber, T. Brief Bioinform. (2017).

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Correspondence to Jason M. Crawford.

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The authors declare no competing interests.

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