BACTERIAL PHYSIOLOGY

CRISPRs from scratch

CRISPR immunity begins with the acquisition of sequences from invading nucleic acids through spacer integration into a CRISPR locus. Off-target integration of spacers into other parts of the genome is now implicated as a spontaneous source of new CRISPR loci.

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Fig. 1: Canonical CRISPR adaptation versus neo-CRISPR genesis.

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Correspondence to Erik J. Sontheimer.

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Edraki, A., Sontheimer, E.J. CRISPRs from scratch. Nat Microbiol 3, 261–262 (2018). https://doi.org/10.1038/s41564-018-0115-9

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