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CRISPRs from scratch

CRISPR immunity begins with the acquisition of sequences from invading nucleic acids through spacer integration into a CRISPR locus. Off-target integration of spacers into other parts of the genome is now implicated as a spontaneous source of new CRISPR loci.

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Fig. 1: Canonical CRISPR adaptation versus neo-CRISPR genesis.


  1. Mohanraju, P. et al. Science 353, aad5147 (2016).

    Article  PubMed  Google Scholar 

  2. Jackson, S. A. et al. Science 356, eaal5056 (2017).

    Article  PubMed  Google Scholar 

  3. Nivala, J., Shipman, S. L. & Church, G. M. Nat. Microbiol. (2018).

  4. Nuñez, J. K., Bai, L., Harrington, L. B., Hinder, T. L. & Doudna, J. A. Mol. Cell 62, 824–833 (2016).

    Article  PubMed  Google Scholar 

  5. Shipman, S. L., Nivala, J., Macklis, J. D. & Church, G. M. Science 353, aaf1175 (2016).

    Article  PubMed  PubMed Central  Google Scholar 

  6. Shipman, S. L., Nivala, J., Macklis, J. D. & Church, G. M. Nature 547, 345–349 (2017).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Nuñez, J. K., Harrington, L. B., Kranzusch, P. J., Engelman, A. N. & Doudna, J. A. Nature 519, 193–198 (2015).

    Article  PubMed  PubMed Central  Google Scholar 

  8. Charpentier, E., Richter, H., van der Oost, J. & White, M. FEMS Microbiol. Rev. 39, 428–441 (2015).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Correspondence to Erik J. Sontheimer.

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Edraki, A., Sontheimer, E.J. CRISPRs from scratch. Nat Microbiol 3, 261–262 (2018).

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